背景:龋齿和牙周炎的病原微生物和临床表现不同,龋齿和牙周炎通常分开讨论,它们之间的关系被忽略。临床上,易于龋齿的患者通常具有更健康的牙周状态,而牙周炎患者的龋齿发生率一般较低。龋齿与牙周炎之间的关系尚不清楚。
目的:本研究旨在通过探索龋齿的生态链和细菌相互作用来解释龋齿与牙周炎拮抗的临床现象。牙周炎,和其他共病。
方法:30例口腔疾病患者的牙菌斑微生物群(10例龋齿,牙周炎,和共病疾病)进行测序,并使用16SrRNA基因测序进行分析。京都基因和基因组百科全书(KEGG)数据库用于龋齿中牙菌斑微生物群落的差异功能分析,牙周炎,和合并症。
结果:合并感染组的牙菌斑细菌丰富度最高。主坐标分析显示龋齿和牙周炎是相互分离的,共病位于龋齿和牙周炎的重叠部分,其中大多数是牙周炎。同时,我们比较了三组之间具有显着差异的微生物组以及微生物组样本之间的相关性。此外,KEGG通路分析显示,三组之间的功能变化存在显着差异。
结论:这项研究揭示了龋齿中牙菌斑微生物群落的组成,牙周炎,合并症和三者之间的差异。此外,我们发现牙周炎和龋齿之间可能存在拮抗作用.我们确定了一种新的治疗策略,用于预测和诊断龋齿和牙周炎。
BACKGROUND: The pathogenic microorganisms and clinical manifestations of caries and periodontitis are different, caries and periodontitis are usually discussed separately, and the relationship between them is ignored. Clinically, patients prone to dental caries generally have a healthier periodontal status, whereas patients with periodontitis generally have a lower incidence of dental caries. The relationship between dental caries and periodontitis remains unclear.
OBJECTIVE: This study aimed to explain the clinical phenomenon of antagonism between dental caries and periodontitis by exploring the ecological chain and bacterial interactions in dental caries, periodontitis, and other comorbid diseases.
METHODS: The dental plaque microbiomes of 30 patients with oral diseases (10 each with caries, periodontitis, and comorbid diseases) were sequenced and analysed using 16 S rRNA gene sequencing. The Kyoto Encyclopaedia of Genes and Genomes (KEGG) database was used for a differential functional analysis of dental plaque microbial communities in caries, periodontitis, and comorbid diseases.
RESULTS: The coinfection group had the greatest bacterial richness in dental plaque. The principal coordinate analysis showed that caries and periodontitis were separate from each other, and comorbid diseases were located at the overlap of caries and periodontitis, with most of them being periodontitis. Simultaneously, we compared the microbiomes with significant differences among the three groups and the correlations between the microbiome samples. In addition, KEGG pathway analysis revealed significant differences in functional changes among the three groups.
CONCLUSIONS: This study revealed the composition of the dental plaque microbial communities in caries, periodontitis, and comorbidities and the differences among the three. Additionally, we identified a possible antagonism between periodontitis and caries. We identified a new treatment strategy for the prediction and diagnosis of caries and periodontitis.