Abstract：
OBJECTIVE: To study the structural characteristics of oral microorganisms in children with caries by 16S rRNA high-throughput sequencing technology.
METHODS: Thirty healthy children aged 3-5 years were enrolled as subjects. According to the index of dmfs, they were divided into caries-free (CF) group (15) and early childhood caries (ECC) group(15). To compare the differences in bacterial community structure, samples of saliva and dental plaque were collected, and high-throughput sequencing was conducted using the Illumina Miseq sequencing platform. Bioinformatics analysis was used to analyze the difference of microbial community structure and diversity with SPSS 23.0 software package.
RESULTS: Microbial diversity in ECC group was significantly lower than CF group. At phylum level, Actinobateria was more abundant in saliva samples of ECC group, while Firmicutes was more abundant in plaque samples of CF group. At genus level, the abundance of Lautropia of CF group was higher in saliva samples while Cardiobacterium, Gemella and Granulicatella were abundant in plaque samples. The abundance of Rothia of ECC group was higher in saliva samples and Corynebacterium was abundant of ECC group in plaque samples.
CONCLUSIONS: There are significant differences in the species and composition of microbial community in saliva and plaque of children with or without caries. Specific microorganisms are related to the occurrence of ECC, and screening specific microorganisms is helpful for early prediction and prevention of ECC.
METHODS: Thirty healthy children aged 3-5 years were enrolled as subjects. According to the index of dmfs, they were divided into caries-free (CF) group (15) and early childhood caries (ECC) group(15). To compare the differences in bacterial community structure, samples of saliva and dental plaque were collected, and high-throughput sequencing was conducted using the Illumina Miseq sequencing platform. Bioinformatics analysis was used to analyze the difference of microbial community structure and diversity with SPSS 23.0 software package.
RESULTS: Microbial diversity in ECC group was significantly lower than CF group. At phylum level, Actinobateria was more abundant in saliva samples of ECC group, while Firmicutes was more abundant in plaque samples of CF group. At genus level, the abundance of Lautropia of CF group was higher in saliva samples while Cardiobacterium, Gemella and Granulicatella were abundant in plaque samples. The abundance of Rothia of ECC group was higher in saliva samples and Corynebacterium was abundant of ECC group in plaque samples.
CONCLUSIONS: There are significant differences in the species and composition of microbial community in saliva and plaque of children with or without caries. Specific microorganisms are related to the occurrence of ECC, and screening specific microorganisms is helpful for early prediction and prevention of ECC.
摘要：
目的：通过16SrRNA高通量测序技术研究龋齿患儿口腔微生物的结构特征。
方法：以30名3～5岁健康儿童为研究对象。根据dmfs的索引，他们被分为无龋齿（CF）组（15）和早期儿童龋齿（ECC）组（15）。为了比较细菌群落结构的差异，收集唾液和牙菌斑样本，使用IlluminaMiseq测序平台进行高通量测序。采用SPSS23.0软件包进行生物信息学分析,分析微生物群落结构和多样性差异。
结果：ECC组微生物多样性显著低于CF组。在门一级，ECC组唾液样本中放线菌含量较高,而Firmicutes在CF组的斑块样品中含量更高。在属一级，CF组唾液样本中Lautropia的丰度较高，而心脏细菌，菌斑样品中含有大量的gemella和Granulicatella。在唾液样品中，ECC组的Rothia丰度较高，而在斑块样品中ECC组的棒状杆菌丰度较高。
结论：有无龋齿儿童的唾液和菌斑中微生物群落的种类和组成存在显着差异。特定的微生物与ECC的发生有关，筛选特定微生物有助于早期预测和预防ECC。
方法：以30名3～5岁健康儿童为研究对象。根据dmfs的索引，他们被分为无龋齿（CF）组（15）和早期儿童龋齿（ECC）组（15）。为了比较细菌群落结构的差异，收集唾液和牙菌斑样本，使用IlluminaMiseq测序平台进行高通量测序。采用SPSS23.0软件包进行生物信息学分析,分析微生物群落结构和多样性差异。
结果：ECC组微生物多样性显著低于CF组。在门一级，ECC组唾液样本中放线菌含量较高,而Firmicutes在CF组的斑块样品中含量更高。在属一级，CF组唾液样本中Lautropia的丰度较高，而心脏细菌，菌斑样品中含有大量的gemella和Granulicatella。在唾液样品中，ECC组的Rothia丰度较高，而在斑块样品中ECC组的棒状杆菌丰度较高。
结论：有无龋齿儿童的唾液和菌斑中微生物群落的种类和组成存在显着差异。特定的微生物与ECC的发生有关，筛选特定微生物有助于早期预测和预防ECC。
