■野生啮齿动物可以作为E.bieneusi的水库或载体,从而使寄生虫传播给家畜和人类。本研究旨在调查中国内蒙古自治区和辽宁省野生啮齿动物中E.bieneusi的流行情况。此外,为了评估基因型水平的人畜共患传播的可能性,对分离株进行了遗传分析.
■从中国两个省份共捕获了486只野生啮齿动物。进行聚合酶链反应(PCR)以扩增啮齿动物粪便DNA中的脊椎动物细胞色素b(cytb)基因,以检测其物种。通过rDNA的内部转录间隔区(ITS)区域的PCR扩增确定E.bieneusi的基因型。遗传特征和人畜共患潜力的检查需要应用相似性和系统发育分析。
■在四种确定的啮齿动物中,E.bieneusi的感染率为5.2%(n=89),黄鲸4.5%(n=96),小家鼠11.3%(n=106),褐家鼠为38.5%(n=195)。在486只啮齿动物中,平均感染率为17.4%。在确定的11种基因型中,已知9个:SHR1(在32个样品中检测到),D(30个样本),EbpA(9个样品),PigEbITS7(8个样品),HNR-IV(6个样品),IV型(5个样品),HNR-VII(2个样品),HNH7(1个样品),和HNPL-V(1个样品)。还发现了两种新的基因型,NMR-I和NMR-II,每个包含一个样本。通过系统发育分析将基因型分为第1组和第13组。
■根据初始报告,E.bieneusi在各自省和地区的野生啮齿动物中非常普遍,并且遗传多样性。这表明这些动物对于E.bieneusi的传播至关重要。携带人畜共患E.bieneusi的动物对当地居民构成重大危害。因此,有必要提高对这些啮齿动物带来的危险的认识,并减少其数量,以防止环境污染。
UNASSIGNED: Wild rodents can serve as reservoirs or carriers of E. bieneusi, thereby enabling parasite transmission to domestic animals and humans. This study aimed to investigate the prevalence of E. bieneusi in wild rodents from the Inner Mongolian Autonomous Region and Liaoning Province of
China. Moreover, to evaluate the potential for zoonotic transmission at the genotype level, a genetic analysis of the isolates was performed.
UNASSIGNED: A total of 486 wild rodents were captured from two provinces in
China. Polymerase chain reaction (PCR) was performed to amplify the vertebrate cytochrome b (cytb) gene in the fecal DNA of the rodents to detect their species. The genotype of E. bieneusi was determined via PCR amplification of the internal transcribed spacer (ITS) region of rDNA. The examination of genetic characteristics and zoonotic potential requires the application of similarity and phylogenetic analysis.
UNASSIGNED: The infection rates of E. bieneusi in the four identified rodent species were 5.2% for Apodemus agrarius (n = 89), 4.5% for Cricetulus barabensis (n = 96), 11.3% for Mus musculus (n = 106), and 38.5% for Rattus norvegicus (n = 195). Infection was detected at an average rate of 17.4% among 486 rodents. Of the 11 identified genotypes, nine were known: SHR1 (detected in 32 samples), D (30 samples), EbpA (9 samples), PigEbITS7 (8 samples), HNR-IV (6 samples), Type IV (5 samples), HNR-VII (2 samples), HNH7 (1 sample), and HNPL-V (1 sample). Two novel genotypes were also discovered, NMR-I and NMR-II, each comprising one sample. The genotypes were classified into group 1 and group 13 via phylogenetic analysis.
UNASSIGNED: Based on the initial report, E. bieneusi is highly prevalent and genetically diverse in wild rodents residing in the respective province and region. This indicates that these animals are crucial for the dissemination of E. bieneusi. Zoonotic E. bieneusi-carrying animals present a significant hazard to local inhabitants. Therefore, it is necessary to increase awareness regarding the dangers presented by these rodents and reduce their population to prevent environmental contamination.