Abstract：
Alveolar echinococcosis (AE), caused by Echinococcus multilocularis, is an important zoonotic disease. Yili Prefecture in Xinjiang is endemic for AE, however the molecular variability of E. multilocularis in this region is poorly understood. In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infected 27.91% (12/43) small rodents. Haplotype network analysis showed that all haplotypes clustered together with the Asian group. Pairwise fixation index (FST) values showed lower level of genetic differentiation between different regions within the country. Compared with the sequences of E. multilocularis from North America and Europe, all concatenated sequences isolated from Yili Prefecture were highly differentiated and formed a single population. The A2 haplotype, analyzed using the cob, nad2, and cox1 genes of E. multilocularis, is the predominant variant in humans and dogs in Yili Prefecture.
摘要：
肺泡包虫病（AE），由多房棘球蚴引起，是一种重要的人畜共患疾病。新疆伊犁州是AE的地方病，然而，该地区多房性大肠杆菌的分子变异性知之甚少。在这项研究中,127个样本用于单倍型分析，包括79个来自人类的组织，43个小啮齿动物的肝脏组织，和5个狗的粪便样本。使用细胞色素b（cob）的线粒体（mt）基因的完整序列研究了多房性大肠杆菌的遗传变异性，NADH脱氢酶亚基2（nad2），和细胞色素c氧化酶亚基1（cox1），每个样品总共使用3558bp。亚洲单倍型2（A2）是显性单倍型，人类患病率为72.15%(57/79)，小啮齿动物占2.33%(1/43)，和狗的80.00%（4/5），其次是A5，第二常见的单倍型，其中感染了27.91%(12/43)的小啮齿动物。单倍型网络分析显示，所有单倍型都与亚洲群体聚集在一起。成对固定指数（FST）值显示该国不同地区之间的遗传分化水平较低。与来自北美和欧洲的多房性大肠杆菌的序列相比，从伊犁地区分离出的所有串联序列均高度分化并形成单个种群。A2单倍型，用棒子分析，多房性大肠杆菌的nad2和cox1基因，是伊犁地区人类和狗的主要变种。
