本研究比较了醋炮制前后姜黄对肝郁气滞型痛经大鼠模型的影响,以揭示醋炮制改善龙骨疏肝止痛作用的机制。根据《痛经动物模型的制备(草案)》和慢性不可预知应激方法,建立肝郁气滞证痛经大鼠模型。体重的变化,器官指数,扭曲延迟,扭动比分,血清6项肝功能指标,性激素,疼痛因素,并测定血液流变学指标,评价食醋处理与否的龙骨治疗肝郁气滞证大鼠痛经的疗效。与模型组相比,C.longa组(是否用醋处理)显示出缓慢的体重减轻,增加扭曲延迟,扭体反应降低(P<0.05)。对生C.longa扭动的抑制率,醋加工的C.longa,阳性组为33.780%,64.611%,和62.466%,分别。食醋处理组的抑制率明显较高,表明食醋处理的龙骨C.longa显示出更显著的治疗效果。醋处理的C.longa组显示较低水平的丙氨酸转氨酶(ALT),碱性磷酸酶(ALP),天冬氨酸转氨酶(AST),直接胆红素(DBIL),总胆红素(TBIL)和白蛋白(ALB)水平较高(P&lt;0.05),表明食醋处理增强了龙眼草对肝损伤的治疗作用。醋处理C.longa组血清雌二醇(E_2)和催产素(OT)水平较低(P&lt;0.05),表明醋加工的C.longa可以调节性激素水平,减少子宫平滑肌的活动和子宫的收缩,减轻大鼠痛经症状。此外,醋处理的C.longa组显示较低的白细胞介素-6(IL-6)和精氨酸加压素(AVP)水平和较高的β-内啡肽(β-EP)水平(P&lt;0.05),这表明醋加工的C.longa调节疼痛因子的水平以发挥缓解疼痛的作用。药物干预降低了全血黏度低切,中切和高切值,血浆粘度,全血还原粘度低切和高切值,红细胞累积压,和红细胞沉降率的方程K值(P<0.05),醋处理的C.longa组优于其他组。该结果表明醋处理增强了C.longa在改善局部血液流变学方面的功能。C.用醋处理的龙骨可以进入肝脏,以减轻对心脏的影响,肝脏,肾,还有子宫,修复肝功能,通过调节性激素和疼痛因子的水平,改善血液流变学,恢复性激素水平和免疫功能。它激活了缓解疼痛的机制来缓解疼痛,保护肝脏,对抗炎症,这与醋加工有助于C.longa进入肝脏以舒缓肝脏和缓解疼痛的理论是一致的。
This study compared the effects of Curcuma longa before and after processing with vinegar on the rat model of dysmenorrhea with the syndrome of liver depression and Qi stagnation to reveal the mechanism of vinegar processing in improving the role of C. longa in soothing liver and relieving pain. The rat model of dysmenorrhea with the syndrome of liver depression and Qi stagnation was established according to the Preparation of the Animal Model of Dysmenorrhea(Draft) and the chronic unpredictable stress me-thod. The changes in the body weight, organ indexes, writhing latency, writhing score, and serum levels of six liver function indicators, sex hormones, pain factors, and blood rheological indicators were measured to evaluate the efficacy of C. longa processed with vinegar or not in treating dysmenorrhea in the rats with syndrome of liver depression and qi stagnation. Compared with the model group, the C. longa group(processed with vinegar or not) showed slow weight loss, increase in writhing latency, and decrease in writhing response(P<0.05). The inhibition rates on writhing in raw C. longa, vinegar-processed C. longa, and positive groups were 33.780%, 64.611%, and 62.466%, respectively. The significantly higher inhibition rate of the vinegar processing group indicated that vinegar-processed C. longa demonstrated more significant therapeutic effect. The vinegar-processed C. longa group showed lower levels of alanine aminotransferase(ALT), alkaline phosphatase(ALP), aspartate aminotransferase(AST), direct bilirubin(DBIL), and total bilirubin(TBIL) and higher level of albumin(ALB)(P<0.05), which indicated that vinegar processing enhanced the therapeutic effect of C. longa on liver injury. The serum levels of estradiol(E_2) and oxytocin(OT) were lower in the vinegar-processed C. longa group(P<0.05), indicating that the vinegar-processed C. longa could regulate the sex hormone levels, reduce the activity of uterine smooth muscle and contraction of uterus, and alleviate the symptoms of dysmenorrhea in rats. Moreover, the vinegar-processed C. longa group showed lower interleukin-6(IL-6) and arginine vasopressin(AVP) levels and higher beta-endorphin(β-EP) level(P<0.05), which indicated that vinegar-processed C. longa regulated the levels of pain factors to exert the pain-relieving effect. Drug intervention decreased the whole blood viscosity low-cut, medium-cut and high-cut values, plasma viscosity, whole blood reduction viscosity low-cut and high-cut values, erythrocyte cumulative pressure, and equation K value of erythrocyte sedimentation rate(P<0.05), and the vinegar-processed C. longa group outperformed other groups. This result indicated that vinegar processing enhanced the function of C. longa in improving the local blood rheology. C. longa processed with vinegar can enter the liver to relieve the da-mage to the heart, liver, kidney, and uterus, repair the liver function, and recover the sex hormone levels and immune function by regulating the levels of sex hormones and pain factors and improving the blood rheology. It activates the pain-relieving mechanism to relieve the pain, protect the liver, and fight inflammation, which is consistent with the theory that vinegar processing facilitates C. longa entering the liver to sooth liver and relieve pain.