黑木耳的栽培,一种可食用的蘑菇,在很大程度上依赖于木材资源的可用性,木材资源是物种生长的基质。为确保海木尔产业的可持续发展,优化玉米芯作为基质的利用,这项研究试图调查玉米芯作为培养A.heimuer的基质的潜在用途。本研究的目的是探索A.heimuer在菌丝体上对玉米芯木质纤维素的利用,原基,和结果阶段,通过专门检查与玉米芯生物质降解相关的碳水化合物活性酶(CAZymes)和差异表达基因(DEGs)的转录组的表达谱。结果显示菌丝体有10,979、10,630和11,061个DEG,原基,和结果阶段,分别,而639个DGE被鉴定为碳水化合物活性酶。特别感兴趣的是与木质纤维素降解直接相关的46种差异表达的CAZymes基因。此外,该研究发现A.heimuer表现出适应性变化,使其能够有效地利用玉米芯中存在的纤维素。这些变化主要在原基和结果阶段观察到。还鉴定了参与木质纤维素降解的关键基因,包括菌丝体阶段的g6952、g8349、g12487和g2976,g5775、g2857、g3018和g11016在原基阶段,和g10290,g2857,g12385,g7656和g8953在结果阶段。本研究发现,裂解多糖单加氧酶(LPMO)在玉米芯纤维素降解中起着至关重要的作用,进一步强调了A.heimuer降解木质纤维素生物质的分子机制的复杂性。该研究揭示了A.heimuer降解玉米芯生物量的分子机制,对木质纤维素资源的有效利用具有重要意义。这项研究的结果可能有助于开发创新的生物技术,以将玉米芯生物质转化为有用的产品。
The cultivation of Auricularia heimuer, a species of edible mushroom, heavily relies on the availability of wood resources serving as substrate for the growth of the species. To ensure the sustainable development of the A. heimuer industry and optimize the utilization of
corncob as a substrate, this study sought to investigate the potential use of
corncob as a substrate for the cultivation of A. heimuer. The purpose of this study was to explore the utilization of
corncob lignocellulose by A. heimuer at the mycelium, primordium, and fruiting stages, by specifically examining the expression profiles of both carbohydrate-active enzymes (CAZymes) and the transcriptome of differentially expressed genes (DEGs) relevant to corncob biomass degradation. The results revealed 10,979, 10,630, and 11,061 DEGs at the mycelium, primordium, and fruiting stages, respectively, while 639 DGEs were identified as carbohydrate-active enzymes. Of particular interest were 46 differentially expressed CAZymes genes that were associated directly with lignocellulose degradation. Furthermore, the study found that A. heimuer exhibited adaptive changes that enabled it to effectively utilize the cellulose present in the
corncob. These changes were observed primarily at the primordium and fruiting stages. Key genes involved in lignocellulose degradation were also identified, including g6952, g8349, g12487, and g2976 at the mycelium stage, g5775, g2857, g3018, and g11016 at the primordium stage, and g10290, g2857, g12385, g7656, and g8953 at the fruiting stage. This study found that lytic polysaccharide monooxygenase (LPMO) played a crucial role in the degradation of corncob cellulose, further highlighting the complexity of the molecular mechanisms involved in the degradation of lignocellulose biomass by A. heimuer. The study sheds light on the molecular mechanisms underlying the ability of A. heimuer to degrade
corncob biomass, with implications for the efficient utilization of lignocellulose resources. The findings from this study may facilitate the development of innovative biotechnologies for the transformation of corncob biomass into useful products.