Abstract：
The short-term composting based on corncob for preparing Pleurotus ostreatus cultivation medium originated from agricultural production practices and so lacked systematic investigation. In this study, the influences of a Dafen (15 mm, DFT) and Xiaofen (5 mm, XFT) initial particle size (IPS) of corncob on the microbial succession and compost quality were examined. Results demonstrated that XFT compost was better suited for mushroom cultivation due to its high biological efficiency of 70 % and the absence of contamination. The composting microbes differed significantly between the DFT and XFT composts. During composting, the genera of Bacillus, Acinetobacter, Lactobacillus, Streptomyces, and Paenibacillus were majorly found in the DFT compost, while Acinetobacter, Lactobacillus, Puccinia, Bacteroides, and Bacillus genera dominated the XFT compost. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that throughout the thermophilic phase, XFT compost had much greater relative abundances of sequences relevant to energy, carbohydrate, and amino acid metabolism than DFT compost. Analysis of network correlations and Mantel tests indicated that IPS reduction could increase microbial interactions. Overall, adjusting the IPS of corncob to 5 mm increased microbial interactions, improved compost quality, and thereby boosted the P. ostreatus yield. These findings will be pertinent in optimizing the composting process of cultivation medium for P. ostreatus.
摘要：
基于玉米芯制备平菇栽培培养基的短期堆肥起源于农业生产实践，因此缺乏系统的调查。在这项研究中,大芬(15毫米，DFT)和小芬(5毫米，XFT)对玉米芯的初始粒径(IPS)对微生物演替和堆肥质量停止了考核。结果表明，由于XFT堆肥具有70％的高生物效率和无污染性，因此更适合蘑菇栽培。DFT和XFT堆肥之间的堆肥微生物差异显着。堆肥过程中,芽孢杆菌属,不动杆菌,乳酸菌,链霉菌,类芽孢杆菌主要存在于DFT堆肥中，而不动杆菌,乳酸菌,Puccinia,拟杆菌,和芽孢杆菌属占主导地位的XFT堆肥。京都基因和基因组百科全书（KEGG）分析表明，在整个嗜热阶段，XFT堆肥具有与能量相关的序列的相对丰度更大，碳水化合物，和氨基酸代谢比DFT堆肥。网络相关性和Mantel测试表明，减少IPS可以增加微生物相互作用。总的来说,将玉米芯的IPS调整到5毫米，增加了微生物的相互作用，提高堆肥质量，从而提高了平菇的产量。这些发现将对优化平菇培养基的堆肥过程具有重要意义。
