三氯生(TCS),三氯卡班(TCC),和氯酚(CPs)是广泛用于皮肤和口腔卫生产品的广谱抗菌药物,这可能会导致严重的肝脏和肠道损伤。因此,建立一种快速、灵敏的TCS监测方法至关重要,TCC,和各种生物体中的CP。在这项工作中,氟官能化共价有机骨架(COF-F)通过使用4,4',4'\'-(1,3,5-三嗪-2,4,6-三基)三苯胺和2,3,5,6-四氟对苯二甲醛作为两个结构单元,并用作固相微萃取(SPME)探针,用于萃取TCS,TCC和CP。COF-F具有优异的疏水性,大的比表面积(1354.3m2g-1)和高均匀孔隙率(3.2nm),这促进了对TCS的高选择性和吸附性能,TCC,和CP。因此,已开发出制备的COF-F-SPME与电喷雾电离质谱相结合,以提供快速,超灵敏的TCS检测,TCC,和生物样品中的CP。所建立的方法对TCS具有令人满意的线性范围(0.01-707μgL-1)和低检出限(0.003-0.040μgL-1),TCC和CP。所开发的方法可以成功地应用于检测TCS,小鼠肝脏和肾脏组织中的TCC和CP,证明了检测生物样品中氯化芳族污染物的潜力。
Triclosan (TCS), triclocarban (TCC), and chlorophenols (CPs) are broad-spectrum antibacterials widely used in dermatological and oral hygiene products, which could induce severe liver and intestine injuries. Hence, it is essential to establish a rapid and sensitive method to monitor TCS, TCC, and CPs in various organisms. In this work, fluorine-functionalized covalent organic framework (COF-F) was prepared by using 4,4\',4\'\'-(1,3,5-triazine-2,4,6-triyl)tri-aniline and 2,3,5,6-tetrafluoroterephthalaldehyde as two building units and employed as a solid phase microextraction (SPME) probe for the extraction of TCS, TCC and CPs. The COF-F possessed excellent hydrophobicity, a large specific surface area (1354.3 m2 g-1) and high uniform porosity (3.2 nm), which facilitated high selectivity and adsorption properties towards TCS, TCC, and CPs. Therefore, the as-prepared COF-F-SPME in combination with electrospray ionization mass spectrometry has been developed to provide fast and ultrasensitive detection of TCS, TCC, and CPs in biological samples. The established method demonstrated satisfactory linear ranges (0.01-100.00 μg L-1) and low limits of detection (0.003-0.040 μg L-1) for TCS, TCC and CPs. The developed method could be successfully applied to detect TCS, TCC and CPs in the liver and kidney tissues of mice, demonstrating the potential for the detection of chlorinated aromatic pollutants in the biological samples.