Abstract：
Triclocarban (TCC), an emerging organic contaminant, poses a potential threat to human health with long-term exposure. Here, Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 were utilized to degrade TCC at environmental related concentrations for enhancing TCC biodegradation and investigating whether the toxicity of intermediate metabolites is lower than that of the parent compound. The results demonstrated that the bacterial consortium could degrade TCC by 82.0% within 7 days. The calculated 96 h LC50 for TCC, as well as its main degradation product 3,4-Dichloroaniline (DCA) were 0.134 mg/L and 1.318 mg/L respectively. Biodegradation also alleviated histopathological lesions induced by TCC in zebrafish liver and gut tissues. Liver transcriptome analysis revealed that biodegradation weakened differential expression of genes involved in disrupted immune regulation and lipid metabolism caused by TCC, verified through RT-qPCR analysis and measurement of related enzyme activities and protein contents. 16 S rRNA sequencing indicated that exposure to TCC led to gut microbial dysbiosis, which was efficiently improved through TCC biodegradation, resulting in decreased relative abundances of major pathogens. Overall, this study evaluated potential environmental risks associated with biodegradation of TCC and explored possible biodetoxification mechanisms, providing a theoretical foundation for efficient and harmless bioremediation of environmental pollutants.
摘要：
三氯卡班(TCC),一种新兴的有机污染物，长期暴露对人类健康构成潜在威胁。这里,红球菌BX2和假单胞菌。LY-1用于在环境相关浓度下降解TCC，以增强TCC的生物降解并研究中间代谢物的毒性是否低于母体化合物的毒性。结果表明，该菌群能在7天内降解TCC82.0%。计算得出的TCC的96小时LC50，以及其主要降解产物3,4-二氯苯胺(DCA)分别为0.134mg/L和1.318mg/L。生物降解还减轻了斑马鱼肝脏和肠道组织中TCC引起的组织病理学损伤。肝脏转录组分析显示，生物降解削弱了TCC引起的免疫调节和脂质代谢破坏基因的差异表达，通过RT-qPCR分析和相关酶活性和蛋白质含量的测定进行验证。16SrRNA测序表明，暴露于TCC导致肠道微生物菌群失调，通过TCC生物降解得到有效改善，导致主要病原体的相对丰度下降。总的来说,这项研究评估了与TCC生物降解相关的潜在环境风险，并探索了可能的生物解毒机制，为环境污染物的高效无害化生物修复提供理论基础。
