目的:通过建立细粒棘球蚴致敏BALB/c小鼠模型,探讨小鼠记忆性T细胞的变化及相关因素。
方法:采用腹腔注射的方法建立致敏BALB/c小鼠模型。对照组(CTRL),非致敏组感染细粒大肠杆菌(CE),并建立了一个感染细粒大肠杆菌(ANPC)的致敏组。小鼠肺组织病理变化,记忆T细胞(CD4Tm)的变化,HE染色检测外周血有核白细胞介素-23(IL-23)的变化,流式细胞术,和液相多蛋白质定量技术,分别。
结果:在CTRL中,小鼠记忆T细胞的个体百分比为9.14±0.45,25.23±0.17和13.29±0.32,CE,和ANPC组,分别。ANPC组的记忆T细胞百分比高于CTRL组(t=18.410,p<.001),但低于CE组(t=-80.147,p<.001)。CTRL中小鼠外周血IL-23水平,CE,ANPC组分别为225.76±27.16、359.21±28.67和215.69±22.69。ANPC组小鼠外周血IL-23水平低于CE组(t=9.609,p<.001),与CTRL组无统计学差异(t=0.697,p=0.502)。
结论:在颗粒大肠杆菌致敏的BALB/c小鼠模型中,外周血中IL-23的表达增加,记忆T细胞增殖并被激活;感染颗粒大肠杆菌的致敏组外周血中IL-23的含量和激活的记忆T细胞数量减少。颗粒大肠杆菌引起的过敏反应与IL-23和记忆T细胞的激活有关。
To investigate the changes in memory T cells and the related factors in mice by the establishment of a BALB/c mouse model of Echinococcus granulosus-induced sensitization.
A sensitized BALB/c mouse model was established by intraperitoneal injection of E. granulosus. A control group (CTRL), a nonsensitized group infected with E. granulosus (CE), and a sensitized group infected with E. granulosus (ANPC) were set up. The pathological changes in lung tissue in mice, the change in memory T cells (CD4 Tm), and the change in peripheral blood nucleated interleukin-23 (IL-23) were detected using HE staining, flow cytometry, and liquid-phase multiple protein quantification techniques, respectively.
The individual percentage of mouse memory T cells was 9.14 ± 0.45, 25.23 ± 0.17, and 13.29 ± 0.32 in the CTRL, CE, and ANPC groups, respectively. The percentage of memory T cells in the ANPC group was higher than that in the CTRL group (t = 18.410, p < .001) but lower than that in the CE group (t = -80.147, p < .001). The levels of IL-23 in peripheral blood of mice in the CTRL, CE, and ANPC groups were 225.76 ± 27.16, 359.21 ± 28.67, and 215.69 ± 22.69, respectively. The level of IL-23 in peripheral blood of mice in the ANPC group was lower than that in the CE group (t = 9.609, p < .001), and there was no statistical difference with the CTRL group (t = 0.697, p = .502).
In the BALB/c mouse model of E. granulosus-induced sensitization, the expression of IL-23 in peripheral blood increased, and the memory T cell proliferated and became activated; there was a decrease in the content of IL-23 in peripheral blood and number of activated memory T cells in the sensitization group infected with E. granulosus. The E. granulosus-induced allergic reaction was related to IL-23 and the activation of memory T cells.