Abstract：
In lab settings, inbred mouse strains like BALB/c, C57BL/6J, and C57BL/6N are commonly used. Research in immunology and infectious diseases indicates that their Th1 and Th2 immune responses differ. However, the specific differences in the immune response to the vaccination still require investigation. In this study, ovalbumin (OVA) was used as an antigen and CpG-enriched recombinant plasmid (pUC18-CpG) as an adjuvant for immunisation. The level of serum-specific antibody IgG was detected by indirect ELISA. At 35dpi, serum cytokine levels were measured using MILLIPLEX®. T lymphocyte clusters from mouse spleen were examined using flow cytometry to investigate the immunological effects of the CPG-OVA vaccine on three different types of mice. The results showed that pUC18-CpG as an adjuvant could successfully enhance the immune response. BALB/c had the highest level of IgG antibody. In the OVA-only group, the CD4+/CD8+ ratio of the three types of mice was generally increased, and the BALB/c group had the highest ratio. After inoculation with CpG-OVA, the CD4+/CD8+ ratio of the three types of mice was lower than that of the OVA-only group, and C57BL/6J was the lowest. Compared with the CpG-OVA group of the three kinds of mice, the levels of Th2 cytokines IL-6 and IL-10 in BALB/c were increased compared with C57BL/6J and C57BL/6N. After OVA, the six cytokines secreted in C57BL/6J were higher than those in the C57BL/6N OVA group. Therefore, C57 is a better model for examining the function of the vaccine in cellular immunity, whereas BALB/c mice are more prone to humoral immunity. In addition to highlighting the CpG plasmid\'s ability to successfully activate the immune response of Th1 and Th2, as well as the expression of IgG in vivo and promote T cell immune typing, this study provides valuable insights into immunology and the selection of mouse models for infectious diseases, providing a valuable resource for designing more effective vaccines in the future.
摘要：
在实验室设置中，近交小鼠品系如BALB/c,C57BL/6J,和C57BL/6N常用。免疫学和感染性疾病的研究表明，它们的Th1和Th2免疫应答不同。然而,对疫苗接种的免疫反应的具体差异仍需要调查。在这项研究中,卵清蛋白（OVA）用作抗原，富含CpG的重组质粒（pUC18-CpG）用作免疫佐剂。间接ELISA法检测血清特异性抗体IgG水平。在35dpi，使用MILLIPLEX®测量血清细胞因子水平。使用流式细胞术检查小鼠脾脏中的T淋巴细胞簇，以研究CPG-OVA疫苗对三种不同类型小鼠的免疫作用。结果表明，pUC18-CpG作为佐剂可以成功增强免疫应答。BALB/c的IgG抗体水平最高。在OVA-only组中,三种小鼠的CD4+/CD8+比值普遍升高,BALB/c组比例最高。接种CpG-OVA后，3种小鼠的CD4+/CD8+比值均低于OVA组,C57BL/6J最低。与CpG-OVA组的三种小鼠比拟,与C57BL/6J和C57BL/6N相比，BALB/c中Th2细胞因子IL-6和IL-10的水平升高。OVA之后,C57BL/6J分泌的6种细胞因子高于C57BL/6NOVA组。因此,C57是检查疫苗在细胞免疫中的功能的更好的模型，而BALB/c小鼠更容易发生体液免疫。除了突出CpG质粒成功激活Th1和Th2的免疫应答以及体内IgG表达和促进T细胞免疫分型的能力外，这项研究为免疫学和传染病小鼠模型的选择提供了有价值的见解，为将来设计更有效的疫苗提供了宝贵的资源。
