背景:有人提出遗传因素可能与变态反应障碍有很大联系。
目的:本研究旨在探讨血清特异性免疫球蛋白E[sIgE],血嗜酸性粒细胞,糖蛋白Ibα基因[GP1BA]rs6065,血小板内皮聚集受体1基因[PEAR1]rs12041331和纤溶酶原激活物抑制剂1基因[PAI-1]rs1799762的多态性。
方法:北京协和医院,这项研究纳入了60名健康参与者和283名过敏性疾病参与者.使用TaqMan-minor沟结合剂[MGB]定量聚合酶链反应[qPCR]检查各组的基因多态性。
结果:TaqMan-MGBqPCR结果与DNA测序结果完全一致,根据该医疗中心的其他研究[Kappa=1,p<0.001]。GP1BArs6065,PEAR1rs12041331和PAI-1rs1799762多态性在过敏患者和健康个体之间没有显示出不同的分布。关于过敏患者,GP1BArs6065的CT[n=33]基因型的血液嗜酸性粒细胞水平高于CC[n=250]基因型[0.59,IQR0.32-0.72vs0.31,IQR0.15-0.61,*109/L,p=0.005]。PEAR1rs12041331的AA[n=46]基因型的血清sIgE低于GA[n=136]和GG[n=101]基因型[GA中位数16.3,IQR3.1-46.3,kU/L,p=0.002;GG中位数12.9,IQR3.0-46.9,kU/L,p=0.003]。PEAR1rs12041331的GA基因型的血嗜酸性粒细胞水平[中位数0.42,IQR0.17-0.74*109/L]高于AA基因型[中位数0.25,IQR0.15-0.41*109/L],p=0.012]。PAI-1rs1799762的5G5G[n=44]基因型的sIgE[中位数5.0,IQR0.1-22.8,kU/L]低于4G5G[n=144][中位数17.3,IQR3.7-46.0,kU/L,p=0.012]。
结论:GP1BArs6065、PEAR1rs12041331和PAI-1rs1799762多态性可能与中国过敏性疾病患者血清sIgE或血液嗜酸性粒细胞的遗传易感性有关。
BACKGROUND: It has been suggested that genetic factors may be substantially linked to allergy disorders.
OBJECTIVE: This study aims to investigate the relationship between the serum specific Immunoglobulin E (sIgE), blood eosinophil, and the polymorphisms of glycoprotein Ib alpha gene (GP1BA) rs6065, platelet endothelial aggregation receptor 1 gene (PEAR1) rs12041331, and plasminogen activator inhibitor 1 gene (PAI-1) rs1799762.
METHODS: From the Peking Union Medical College Hospital, this study enrolled 60 healthy participants and 283 participants with allergic diseases. TaqMan-minor groove binder (MGB) quantitative polymerase chain reaction (qPCR) was used to examine the gene polymorphisms in each group.
RESULTS: The TaqMan-MGB qPCR results were completely consistent with the DNA sequencing results, according to other studies in this medical center (Kappa =1, p <0.001). The GP1BA rs6065, PEAR1 rs12041331, and PAI-1 rs1799762 polymorphisms did not show different distribution between allergy patients and healthy individuals. Concerning allergy patients, the CT (n=33) genotype of GP1BA rs6065 had higher blood eosinophil level than the CC (n=250) genotype (0.59, IQR 0.32-0.72 vs 0.31, IQR 0.15-0.61, *109/L, p =0.005). The serum sIgE of AA (n=46) genotype of PEAR1 rs12041331 was lower (median 3.7, interquartile quartiles (IQR) 0.2-16.8, kU/L) than the GA (n=136) and GG (n=101) genotypes (GA median 16.3, IQR 3.1-46.3, kU/L, p = 0.002; GG median 12.9, IQR 3.0-46.9, kU/L, p =0.003). The GA genotypes of PEAR1 rs12041331were with higher blood eosinophil levels (median 0.42, IQR 0.17-0.74 *109/L) than the AA genotype (median 0.25, IQR 0.15-0.41*109/L, p =0.012). The sIgE of the 5G5G (n=44) genotype of PAI-1 rs1799762 was lower (median 5.0, IQR 0.1-22.8, kU/L) than the 4G5G (n=144) (median 17.3, IQR 3.7-46.0, kU/L, p = 0.012).
CONCLUSIONS: The GP1BA rs6065, PEAR1 rs12041331, and PAI-1 rs1799762 polymorphisms may be associated with the genetic susceptibility of serum sIgE or blood eosinophil in Chinese allergic disease patients.