白僵菌(Bal。-Criv.)是一种重要的昆虫病原真菌,用于管理全球各种农业害虫。然而,所有的菌株都可能对白虱没有效果,烟粉虱,或其他害虫,菌株在生长中表现出多样性,孢子形成,毒力特征,和整体生物功效。因此,选择最有效的菌株,需要设计一个全面的方法。我们研究了从19种昆虫中分离出的102个菌株之间的多样性。毒力,和分子系统发育,为塔巴奇的管理确定有前途的。菌株在菌丝生长中表现出多样性,分生孢子生产,以及它们对B.tabaci若虫的毒力。最高的若虫死亡率(2龄和3龄)记录与MTCC-4511(95.1%),MTCC-6289(93.8%),和MTCC-4565(89.9%),浓度为1×106分生孢子ml-1。最高的生物功效指数(BI)是MTCC-4511(78.3%),MTCC-4565(68.2%),和MTCC-4543(62.1%)。MTCC-4511,MTCC-4565和MTCC-4543的前两个主成分的特征值呈正负载,聚类分析也与PCA(主成分分析)(若虫死亡率和BI)非常吻合。分子系统发育无法得出生理特征之间的任何明显关系,菌株的毒力与宿主和位置。BI,PCA,和平方欧氏距离簇被发现是选择潜在昆虫病原菌株的最有用工具。通过开发有效的制剂,所选择的菌株可用于田间烟粉虱若虫种群的管理。关键点:•102株对烟粉虱的生长和毒力表现出多样性。•生物功效指数,PCA,和SED组是选择潜在菌株的有效工具。•选择MTCC-4511、4565和4543作为杀死粉虱若虫的最强毒株。
Beauveria bassiana (Bal.-Criv.) is an important entomopathogenic fungus being used for the management of various agricultural pests worldwide. However, all strains of B. bassiana may not be effective against whitefly, Bemisia tabaci, or other pests, and strains show diversity in their growth, sporulation,
virulence features, and overall bioefficacy. Thus, to select the most effective strain, a comprehensive way needs to be devised. We studied the diversity among the 102 strains of B. bassiana isolated from 19 insect species based on their physiological features,
virulence, and molecular phylogeny, to identify promising ones for the management of B. tabaci. Strains showed diversity in mycelial growth, conidial production, and their
virulence against B. tabaci nymphs. The highest nymphal mortality (2nd and 3rd instar) was recorded with MTCC-4511 (95.1%), MTCC-6289 (93.8%), and MTCC-4565 (89.9%) at a concentration of 1 × 106 conidia ml-1 under polyhouse conditions. The highest bioefficacy index (BI) was in MTCC-4511 (78.3%), MTCC-4565 (68.2%), and MTCC-4543 (62.1%). MTCC-4511, MTCC-4565, and MTCC-4543 clustered with positive loading of eigenvalues for the first two principal components and the cluster analysis also corresponded well with PCA (principal component analysis) (nymphal mortality and BI). The molecular phylogeny could not draw any distinct relationship between physiological features, the
virulence of B. bassiana strains with the host and location. The BI, PCA, and square Euclidean distance cluster were found the most useful tools for selecting potential entomopathogenic strains. The selected strains could be utilized for the management of the B. tabaci nymphal population in the field through the development of effective formulations. KEY POINTS: • 102 B. bassiana strains showed diversity in growth and
virulence against B. tabaci. • Bioefficacy index, PCA, and SED group are efficient tools for selecting potential strains. • MTCC-4511, 4565, and 4543 chosen as the most virulent strains to kill whitefly nymphs.