METHODS: The sub-MICs of CIP were selected using the growth curve approach. The untreated and treated isolates with sub-MICs of CIP were assessed for their biofilm development, motilities on agar, and other virulence factors. The cell morphology of untreated and treated isolates with sub-MIC of CIP was explored using electron microscope. Moreover, the expression levels of the virulence genes in isolates were measured using quantitative real-time PCR.
RESULTS: Data revealed that sub-MICs of CIP significantly (p < 0.05), in a concentration-dependent manner, inhibited biofilm formation and other virulence factors in the selected isolates. Electron microscope analysis showed cell enlargement and various abnormalities in the cell wall and membrane integrity.
CONCLUSIONS: Sub-MICs of CIP exhibited inhibition of virulence and alterations in morphological integrity against P. mirabilis isolates.
方法:使用生长曲线方法选择CIP的亚MIC。评估了未经处理和处理的具有CIP亚MIC的分离株的生物膜发育,琼脂上的动症,和其他毒力因子。使用电子显微镜探索了未经处理和处理的具有CIP亚MIC的分离株的细胞形态。此外,使用定量实时PCR测量分离株中毒力基因的表达水平。
结果:数据显示CIP的亚MIC显着(p<0.05),以浓度依赖的方式,在选定的分离物中抑制生物膜的形成和其他毒力因子。电子显微镜分析显示细胞增大以及细胞壁和膜完整性的各种异常。
结论:CIP的亚MIC表现出针对奇异假单胞菌分离株的毒力抑制和形态完整性改变。