本研究旨在调查抗生素耐药基因CTX-M和Qnr的流行情况。以及毒力基因HlyA,爸爸,CNF1和Afa,在来自埃及人口的尿路致病性大肠杆菌(UPEC)分离物中。在这项横断面研究中,从2020年12月至2021年11月坦塔大学医院收治的尿路感染(UTI)患者的尿液样本中,共收集了50株大肠杆菌.分离株被培养,已识别,并通过圆盘扩散法测试抗生素敏感性。CTX-M,Qnr(QnrA,B,和S),爸爸,CNF1,HlyA,通过聚合酶链反应检测UPEC分离株中的Afa基因。ThePap,CNF1,HlyA,发现18%的Afa基因为阳性,12%,10%,2%的分离株,分别。此外,发现CTX-M和QnrS在44%和8%的分离株中呈阳性,而未检测到QnrA和B。此外,pap阳性,CNF1和HlyA基因与上部和下部UTI显著相关,频率增加,紧迫性,和排尿困难,和复杂的UTI,以及每个高功率视野超过100个白细胞的脓尿。总之,毒力和抗生素抗性基因的流行程度因人群而异。在我们的医院,Pap基因是最普遍的毒力基因,与复杂的UTI密切相关,而CTX-M和QnrS基因最普遍,与抗生素耐药性相关。我们的发现,然而,由于样本量小,应谨慎解释。
This
study aimed to investigate the prevalence of antibiotic resistance genes CTX-M and Qnr, as well as the virulence genes HlyA, Pap, CNF1, and Afa, in uropathogenic Escherichia coli (UPEC) isolates from the Egyptian population. In this cross-sectional
study, a total of 50 E. coli isolates were collected from urine samples from patients with urinary tract infections (UTIs) admitted to Tanta University Hospital from December 2020 to November 2021. The isolates were cultured, identified, and tested for antibiotic susceptibility by the disc diffusion method. The CTX-M, Qnr (QnrA, B, and S), Pap, CNF1, HlyA, and Afa genes were detected by polymerase chain reaction in UPEC isolates. The Pap, CNF1, HlyA, and Afa genes were found to be positive in 18%, 12%, 10%, and 2% of the isolates, respectively. In addition, CTX-M and QnrS were found to be positive in 44% and 8% of the isolates, while QnrA and B were not detected. Furthermore, positive Pap, CNF1, and HlyA genes were significantly associated with both upper and lower UTIs, increased frequency, urgency, and dysuria, and complicated UTIs, as well as pyuria over 100 white blood cells per high-power field. In conclusion, the prevalence of virulence and antibiotic resistance genes varies by population. At our hospital, the Pap gene is the most prevalent virulence gene and was strongly associated with complicated UTIs, while the CTX-M and QnrS genes were the most prevalent and related to antibiotic resistance. Our findings, however, should be interpreted with caution due to the small sample size.