尿路感染是人类最常见的细菌性疾病之一，和大肠杆菌是最常见的相关病原体。大肠杆菌的特定病理类型，被称为尿路致病性大肠杆菌(UPEC)，通常会导致严重且难以治疗的尿路感染。我们提出了一种新的单管筛选工具，该工具使用（N）（6）（CGG）（4）引物来生成指纹图谱，从而可以对该组细菌进行快速区分和流行病学。我们在127个大肠杆菌菌株中发现了71个不同的CGG-PCR图谱，而同一组的肠杆菌重复基因间共识（ERIC）-PCR仅产生28个谱。此外,（CGG）（4）为基础的PCR测试被证明是非常有效的聚类UPEC菌株表现出多个毒力基因，通常属于B2系统发育组，它从缺乏大多数UPEC特异性毒力因子的大肠杆菌菌株中分离出这些菌株。由于CGG-PCR筛选的可重复性高于ERIC-PCR,我们的测试应该是提高当前UPEC打字方案辨别能力的一种有价值的手段。
Urinary tract infections are one of the most frequent bacterial diseases in humans, and Escherichia coli is most often the relevant pathogen. A specific pathotype of E. coli, known as uropathogenic E. coli (UPEC), often causes serious and difficult-to-treat infections of the urinary tract. We propose a new single-tube screening tool that uses an (N)(6)(CGG)(4) primer to generate fingerprint profiles that allow rapid discrimination and epidemiology of this group of bacteria. We found 71 different CGG-PCR profiles among 127 E. coli strains, while enterobacterial repetitive intergenic
consensus (ERIC)-PCR of the same group yielded only 28 profiles. Additionally, the (CGG)(4)-based PCR test turned out to be very effective for clustering UPEC strains exhibiting multiple virulence genes and usually belonging to the B2 phylogenetic group, and it separated these strains from E. coli strains lacking most of the UPEC-specific virulence factors. Since the reproducibility of the CGG-PCR screen is higher than that of ERIC-PCR, our test should be a valuable means of increasing the discriminatory power of current UPEC typing schemes.
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