表面增强拉曼光谱(SERS)技术是一种能够获得分析物指纹信息的有效分析技术,可以在单分子水平上提供检测极限性能,和分析在单个步骤中执行,没有任何中间步骤。SERS技术提供了额外的好处,而不是其他分析技术,包括高选择性,超灵敏检测,简单的协议,就地取样,投入能力和成本效益。由于材料和纳米技术科学的发展与SERS分析技术相结合,这项技术日益增强其使用优势。限制该技术使用的最重要因素是将含有所需分析物的溶液滴到SERS基底上,并且在随后的分析中不能再使用相同的基底。为了解决这个问题,近年来,科学家们一直致力于开发可重复使用的SERS基板。在这些研究中,科学家基本上使用了三种SERS基底清洁应用(1)用合适的溶剂洗涤SERS基底,该溶剂可以在分析后从SERS表面洗脱分析物,(2)在分析之后通过用催化活性材料对分析物进行改性,用分析物的催化降解来清洁SERS基底,和(3)在分析之后对SERS基底应用等离子体清洁程序,和(4)在SERS分析之前应用吸附和解吸程序。在这里,这篇评论文章的目的是根据其开发水平和回收潜力来评估可重复使用的SERS基底方法。这篇综述提供了关于制造SERS基板所采用的各种感测方案的连贯讨论。我们采用了一种关键方法,其中选择了详尽的示例来突出各种实验配置的关键缺点。同样,讨论了有关关键仪器进步的优点和局限性,以及该领域开发的最新方法的扩展。
Surface Enhanced Raman Spectroscopy (SERS) technique is an effective analytical technique in which fingerprint information about analytes can be obtained, can provide detection limit performance at the single molecule level, and analyzes are performed in a single step without any intermediate steps. SERS technique offers additional benefits rather than other analytical techniques including high selectivity, ultrasensitive detection, uncomplicated protocols, in situ sampling, on-set capability and cost-effectiveness. As a result of the combination of developments in materials and nanotechnology science with the SERS analysis technique, this technique strengthens its use advantage day by day. The most important factor that limited the use of this technique was the fact that the solution containing the desired analyte(s) was dropped onto the SERS substrate and the same substrate could not be reused in subsequent analyses. To solve this problem, scientists have focused on developing reusable SERS substrates in recent years. In these studies, scientists basically used three SERS substrate cleaning applications (1) washing the SERS substrate with a suitable solvent that can elute the analyte from SERS surface after analysis, (2) cleaning the SERS substrate with catalytic degradation of analytes after analysis by modifying them with catalytic active materials and (3) Applying plasma cleaning procedure to SERS substrate after analysis and (4) applying adsorption and desorption procedure prior to SERS analysis. Herein, the aim of this review article is to evaluate the reusable SERS substrates-based methods based on their level of development and their potential to recycle. This review offers a coherent discussion on a wide range of sensing schemes employed in fabricating the SERS substrates. We utilized a critical approach in which elaborative examples were selected to highlight key shortcomings of various experimental configurations. In the same vein, there is a discussion of the advantages and limitations concerning the key instrumental advances and the expansion of the recent methods developed in this area.