Capnocytophagacanimorsus和Capnocytophagacynodegmi是狗口腔中的共生细菌。两者都是人畜共患病原体,可以通过狗咬伤感染人类。C.canimorsus可能会导致危及生命的感染,而C.cynodegmi感染倾向于更温和和更局部化。canimorsus的囊状血清A-C似乎与毒力相关。迄今为止描述的一些C.canimorsus血清型也可以在其他Capnocytophaga物种中检测到,包括C.cynodegmi。这项初步研究的目的是调查幼犬口腔出生后C.canimorsus和C.cynodegmi的出现,并评估大坝的Capnocytophagaspp的影响。承运人地位的出现。十窝,共59只小狗,包括在研究中。幼犬和他们的水坝在七个星期后的五个时间点被采样。通过物种特异性聚合酶链反应(PCR)研究了口腔拭子样品中是否存在C.canimorsus和C.cynodegmi,通过对PCR产物的选择进行测序来验证其特异性。在CapnocytophagaPCR反应中呈阳性的样品也通过PCR进行胶囊分型,以获得有关Capnocytophagaspp的更多知识。存在于样品中。总共有10.2%和11.9%的小狗,或20.0%和30.0%的产仔被检测为canimorsus和cynodegmi的PCR阳性,分别。CapnocytophagaPCR阳性的小狗样本始终仅对C.cynodegmi或C.canimorsus呈阳性,不是两者。大多数CapnocytophagaPCR阳性幼犬在5至7周龄时变为阳性。只有少数(5/16)的C.cynodegmiPCR阳性犬样品在胶囊分型PCR中呈阳性,而所有canimorsusC.canimorsusPCR阳性的狗样品在荚膜分型PCR中均为阴性。对于所有CapnocytophagaPCR阳性幼犬,他们的水坝对相同的Capnocytophaga物种呈阳性。这些结果表明,在乳牙萌出时,幼犬从其大坝中被C.cynodegmi或C.canimorsus定殖。
Capnocytophaga canimorsus and Capnocytophaga cynodegmi are commensal bacteria in the oral cavities of dogs. Both are zoonotic pathogens that could infect humans via dog bites. C. canimorsus may cause life-threatening infections in humans, whereas C. cynodegmi infections tend to be milder and more localized. Capsular serovars A-C of C. canimorsus seem to be virulence-associated. Some of the C. canimorsus serovars described to date can also be detected in other Capnocytophaga species, including C. cynodegmi. The objective of this pilot
study was to investigate the emergence of C. canimorsus and C. cynodegmi after birth in oral cavities of puppies and to evaluate the impact of the dam\'s Capnocytophaga spp. carrier status on the emergence. Ten litters, altogether 59 puppies, were included in the
study. The puppies and their dams were sampled at five time points over seven weeks after whelping. Oral swab samples taken were investigated for the presence of C. canimorsus and C. cynodegmi by species-specific polymerase chain reaction (PCR), the specificity of which was verified by sequencing a selection of the PCR products. Samples that were positive in Capnocytophaga
PCR reactions were also capsular-typed by
PCR to gain more knowledge about the Capnocytophaga spp. present in the samples. Altogether 10.2% and 11.9% of puppies, or 20.0% and 30.0% of litters tested PCR-positive for C. canimorsus and C. cynodegmi, respectively. Capnocytophaga PCR-positive puppy samples were always positive for only C. cynodegmi or C. canimorsus, not both. Most Capnocytophaga
PCR-positive puppies became positive at the age of 5 to 7 weeks. Only a minority (5/16) of the C. cynodegmi PCR-positive dog samples were positive in capsular typing
PCR, whereas all C. canimorsus
PCR-positive dog samples were negative in capsular typing
PCR. For all Capnocytophaga PCR-positive puppies, their dam was positive for the same Capnocytophaga species. These results suggest that puppies become colonized by C. cynodegmi or C. canimorsus from their dams at the time of deciduous teeth eruption.