PDF(Pubmed)
Abstract：
Several pathotypes of enteric E. coli have been identified. The group represented by Shiga toxin-producing E. coli (STEC) is of particular interest. Raw milk and raw milk products are significant sources of STEC infection in humans; therefore, identifying pathogens at the herd level is crucial for public health. Most national surveillance programs focus solely on raw milk and raw milk cheeses that are ready for retail sale, neglecting the possibility of evaluating the source of contamination directly at the beginning of the dairy chain. To assess the viability of the application of new molecular methodologies to STEC identification in raw milk filters and in calf feces, we analyzed 290 samples from 18 different dairy herds, including 88 bulk tank milk (BTM), 104 raw milk filters (RMF), and 98 calf feces samples. In total 3.4% of BTM, 41.4% of RMF, and 73.4% of calves\' feces were positive for stx, supporting our hypothesis that BTM is not a suitable matrix to assess the presence of STEC at herd level, underestimating it. Our conclusion is that the surveillance program needs critical and extensive improvements such as RMF and calves\' feces analysis implementation to be more efficient in detecting and preventing STEC infections. The epidemiology of these infections and the characteristics of the pathogen clearly show how a One Health approach will be pivotal in improving our capabilities to control the spread of these infections.
摘要：
已经鉴定了几种肠大肠杆菌的致病型。由产志贺毒素的大肠杆菌(STEC)代表的组是特别令人感兴趣的。原料奶和生奶制品是人类STEC感染的重要来源；因此，在畜群水平上识别病原体对公共卫生至关重要。大多数国家监测项目只关注可零售的原料奶和原料奶奶酪，忽略了在乳品链开始时直接评估污染源的可能性。为了评估将新分子方法应用于原奶过滤器和小牛粪便中的STEC鉴定的可行性，我们分析了来自18个不同奶牛群的290个样本，包括88个散装罐式牛奶(BTM)，104原奶过滤器（RMF），和98个小牛粪便样本.总共占BTM的3.4%，41.4%的RMF，73.4%的小牛粪便对stx呈阳性，支持我们的假设，即BTM不是评估群体水平STEC存在的合适矩阵，低估了它。我们的结论是，监测计划需要关键和广泛的改进，如RMF和小牛粪便分析实施，以更有效地检测和预防STEC感染。这些感染的流行病学和病原体的特征清楚地表明，“一个健康”方法将如何提高我们控制这些感染传播的能力。
