临床决策和生物医学研究在很大程度上依赖于成像技术来可视化组织形态。为了详细检查组织,有必要使用特殊的组织化学染色来增强对比度。这项荟萃分析旨在评估这些染色在诊断口腔病理标本中的敏感性和特异性。我们在8个数据库中进行了搜索,包括EMBASE,PubMed,WebofScience,Scopus,ProQuest,奥维德,Cinahl,还有Cochrane,到2022年6月。在87,393项研究中,我们选择了41篇文章纳入我们的研究。结果表明,特殊组织化学染色的特异性和敏感性分别为86%,置信区间(CI)95%:80%-90%和83%,CI95%:75%-89%,分别。在评估的污渍中,甲苯胺蓝,巴帕尼科洛,银染,Giemsa,克,Feulgen,高碘酸希夫(PAS)是最常用的恶性肿瘤检测,癌前病变,发育不良,和念珠菌病。分别分析每种染色的特异性和敏感性,考虑到标本的类型。甲苯胺蓝是最常用的特殊组织化学染色,特别有效,为了检测恶性肿瘤,CI95%:88%-99%的特异性为97%,CI95%:56%-89%的敏感性为76%。总之,特殊的组织化学染色可有效诊断口腔病变,表现出合理的特异性和敏感性,尤其是在癌前病变和恶性病变的情况下。根据我们研究中的评论文章,银染被鉴定为高度敏感,而Giemsa和Papanicolaou染色表现出最高的特异性。
Clinical decision-making and biomedical research heavily rely on imaging techniques to visualize tissue morphology. To examine tissues in detail, it is necessary to use special histochemical stains to enhance contrast. This meta-analysis aimed to assess the sensitivity and specificity of these stains in diagnosing oral pathologic specimens. We conducted a search in 8 databases, including EMBASE, PubMed, Web of Science, Scopus, ProQuest, Ovid, Cinahl, and Cochrane, up to June 2022. Of 87,393 studies, 41 articles were selected for inclusion in our study. The results revealed that the specificity and sensitivity of the special histochemical stains were 86% with confidence interval (CI) 95%: 80%-90% and 83% with CI 95%: 75%-89%, respectively. Among the stains evaluated, toluidine blue, Papanicolaou, silver stain, Giemsa, Gram, feulgen, and periodic acid-Schiff (PAS) were the most frequently used for the detection of malignancy, premalignant lesions, dysplasia, and candidiasis. The specificity and sensitivity of each stain were analyzed individually, considering the type of specimen. Toluidine blue was the most commonly utilized special histochemical stain, particularly effective, for detecting malignancy, with a specificity of 97% with CI 95%: 88%-99% and sensitivity of 76% with CI 95%: 56%-89%. In conclusion, special histochemical stains are effective in diagnosing oral lesions, exhibiting reasonable specificity and sensitivity, especially in cases of premalignant and malignant lesions. Based on the reviewed articles in our study, the silver stain was identified as highly sensitive, while Giemsa and Papanicolaou stain exhibited the highest specificity.