目的:运动对预防肝癌是有效的。运动通过调节microRNAs(miRNAs)和细胞因子/肌肉因子发挥生物学作用。我们旨在研究低强度抗阻运动对无运动习惯的受试者血清miRNA和细胞因子/肌肉因子表达的影响。
方法:在这项前瞻性前后研究中,我们招募了7名没有运动习惯的男性受试者。所有受试者都进行了低强度阻力运动计划(三种代谢当量,大约20分钟/会话)。使用微阵列评估血清miRNA表达。我们对运动前后差异表达的miRNA进行了京都基因和基因组百科全书(KEGG)途径富集分析。使用多重小组评估血清细胞因子/肌动蛋白表达。
结果:所有受试者均完成了锻炼计划,无不良事件。在微阵列分析中,7个miRNAs在运动前后表现出显著的变化。其中,microRNA(miR)-630和miR-5703显示>1.5倍的增加(miR-630:40.7vs.69.3信号强度,p=0.0133;miR-5703:30.7vs.55.9信号强度,p=0.0051)。KEGG通路富集分析表明,miR-630-和miR-5703相关基因在37和5个通路中富集,包括转化生长因子-β和Wnt信号通路,分别。在多元分析中,运动后与运动前相比,有12种细胞因子/肌细胞因子显示出明显的变化。其中,Fractalkine/CX3CL1通过运动显示出最显着的上调(94.5vs.109.1pg/ml,p=0.0017)。
结论:在没有运动习惯的受试者中,低强度抗阻运动计划与血清miR-630、miR-5703和fractalkine/CX3CL1表达上调相关。因此,即使是低强度运动也可能改变人体内miRNA和细胞因子/Myokine的表达。
OBJECTIVE: Exercise is effective for the prevention of liver cancer. Exercise exerts biological effects through the regulation of microRNAs (miRNAs) and cytokines/myokines. We aimed to investigate the effects of low-intensity resistance exercise on serum miRNA and cytokine/
myokine expressions in subjects with no exercise habits.
METHODS: We enrolled seven male subjects with no exercise habits in this prospective before-after
study. All subjects performed a low-intensity resistance exercise program (three metabolic equivalents, approximately 20 min/session). Serum miRNA expressions were evaluated using microarrays. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed miRNAs before and after exercise. Serum cytokine/
myokine expressions were evaluated using a multiplex panel.
RESULTS: All subjects completed the exercise program with no adverse events. In the microarray analysis, seven miRNAs showed a significant change between before and after exercise. Of these, microRNA (miR)-630 and miR-5703 showed a >1.5-fold increase (miR-630: 40.7 vs. 69.3 signal intensity, p = 0.0133; miR-5703: 30.7 vs. 55.9 signal intensity, p = 0.0051). KEGG pathway enrichment analysis showed that miR-630- and miR-5703-related genes were enriched in 37 and 5 pathways, including transforming growth factor-beta and Wnt signaling pathways, respectively. In the multiplex analysis, 12 cytokines/myokines showed significant alteration after exercise compared to before exercise. Of these, fractalkine/CX3CL1 showed the most significant up-regulation by exercise (94.5 vs. 109.1 pg/ml, p = 0.0017).
CONCLUSIONS: A low-intensity resistance exercise program was associated with upregulation of serum miR-630, miR-5703, and fractalkine/CX3CL1 expressions in subjects with no exercise habits. Thus, even low-intensity exercise may alter miRNA and cytokine/
myokine expressions in humans.