电感耦合等离子体质谱及其同位素富集标准品制备方法的进展,允许通过同位素稀释质谱法高精度测量金属蛋白。这种技术现在已经达到了成熟的水平,即精度的阶跃变化,精度,和可追溯性,特别是,临床,生物医学测量是可以实现的。目前的临床测量,这需要在复杂样品基质存在下的低检测限,使用基于免疫化学的间接方法研究人类疾病。然而,这种方法的可追溯性差,要求在提供基于矩阵的参考材料的基础上进行比较,用作分析标准。这导致当需要改变参考材料时的困难,通常导致临床结果和参考范围缺乏实验室间和时间可比性。在这次审查中,我们专注于临床研究中最重要的金属蛋白,为了说明色谱与无机质谱联用的属性如何用于直接测量金属蛋白如血红蛋白,转铁蛋白,和铜蓝蛋白.通过使用这种方法,我们希望展示同位素稀释分析如何作为参考方法,以提高可追溯性和支持临床,生物医学,和其他生物测量。
Advances in inductively coupled plasma mass spectrometry and the methods used to prepare isotopically enriched standards, allow for the high accuracy measurement of
metalloproteins by isotope dilution mass spectrometry. This technique has now reached a level of maturity whereby a step change in the accuracy, precision, and traceability of, in particular, clinical, and biomedical measurements is achievable. Current clinical measurements, which require low limits of detection in the presence of complex sample matrices, use indirect methods based on immunochemistry for the study of human disease. However, this approach suffers from poor traceability, requiring comparisons based on provision of matrix-based reference materials, used as analytical standards. This leads to difficulty when changes in the reference material are required, often resulting in a lack of interlaboratory and temporal comparability in clinical results and reference ranges. In this
review, we focus on the most important
metalloproteins for clinical studies, to illustrate how the attributes of chromatography coupled to inorganic mass spectrometry can be used for the direct measurement of
metalloproteins such as hemoglobin, transferrin, and ceruloplasmin. By using this approach, we hope to demonstrate how isotope dilution analysis can be used as a reference method to improve traceability and underpin clinical, biomedical, and other biological measurements.