■涉及福尔马林固定的直接免疫荧光值的数据,石蜡包埋组织(IF-石蜡)在肾脏疾病的诊断是有争议的。这项研究的目的是研究在应用IF-石蜡技术时,通过常规免疫荧光对冷冻组织(IF-Frozen)进行评估的肾脏活检是否会产生足够的发现以确认诊断。
■为了显示免疫球蛋白,补体成分,和轻链,对55例天然肾活检进行IF-石蜡和IF-冷冻染色技术。比较染色的强度,并计算灵敏度和特异度。
■IF-石蜡技术显示出89%的灵敏度,81%,86%,30%,71%,60%,IgG为77%,IgM,IgA,C1q,C3,κ,和λ,分别,而特异性为91%,100%,100%,96%,94%,98%,和100%。它显示了87%的病例的诊断结果。与同时使用IF-石蜡和IF-冷冻染色技术的病例相比,55个中的43个显示诊断免疫球蛋白/补体的强度相等或稍有差异。
■福尔马林固定的直接免疫荧光,石蜡包埋切片不能代替免疫荧光冷冻切片在肾活检的评估,但当冷冻组织不足或不可用时,可能是一种“抢救技术”,必须非常谨慎地解释。
UNASSIGNED: The data referring to the value of direct
immunofluorescence on formalin-fixed, paraffin-embedded tissue (IF-Paraffin) in the diagnosis of renal diseases is controversial. The aim of this
study was to investigate whether renal biopsies evaluated by routine
immunofluorescence on frozen tissue (IF-Frozen) would yield adequate findings to confirm diagnoses when the IF-Paraffin technique was applied.
UNASSIGNED: To show immunoglobulins, complement components, and light chains, 55 native renal biopsies were subjected to IF-Paraffin and IF-Frozen staining techniques. The intensity of the staining was compared, and the sensitivity and specificity were calculated.
UNASSIGNED: The IF-Paraffin technique showed a sensitivity of 89%, 81%, 86%, 30%, 71%, 60%, and 77% for IgG, IgM, IgA, C1q, C3, κ, and λ, respectively, whereas specificity was 91%, 100%, 100%, 96%, 94%, 98%, and 100%. It showed diagnostic findings in 87% of cases. Compared to cases that had both IF-Paraffin and IF-Frozen staining techniques, 43 of 55 showed either equal intensity for the diagnostic immunoglobulin/complement or a little difference.
UNASSIGNED: Direct
immunofluorescence on formalin-fixed, paraffin-embedded sections cannot replace
immunofluorescence on frozen sections in the assessment of renal biopsies, but may be a \"salvage technique\" when frozen tissue is insufficient or unavailable and must be interpreted with great caution.