METHODS: We evaluated the immune contexture of 6 BC and 5 LADC MPEs and their PTs using 3 multiplex immunofluorescence panels. We explored the associations between sample characteristics and pleural effusion-free survival.
RESULTS: No MPE samples had positive programmed death-ligand 1 expression in malignant cells, although 3 of 11 PTs has positive programmed death-ligand 1 expression (more than 1% expression in malignant cells). Overall, in LADC samples, cluster of differentiation 3 (CD3)+ T cells and CD3+CD8+ cytotoxic T cells predominated (median percentages for MPEs versus PTs: 45.6% versus 40.7% and 4.7% versus 6.6%, respectively) compared with BC. CD68+ macrophages predominated in the BC samples (medians for MPEs 61.2% versus PTs for 57.1%) but not in the LADC samples. Generally in PTs, CD3+CD8+ forkhead box P3+ T cells and the median distances from the malignant cells to CD3+CD8+Ki67+ and CD3+ programmed cell death protein 1 + T cells correlated to earlier MPE after PT diagnosis.
CONCLUSIONS: The immune cell phenotypes in the MPEs and PTs were similar within each cancer type but different between BC versus LADC. An MPE analysis can potentially be used as a substitute for a PT analysis, but an expanded study of this topic is essential.
方法:我们使用3个多重免疫荧光小组评估了6BC和5个LADCMPE及其PT的免疫背景。我们探讨了样本特征与无胸腔积液生存率之间的关系。
结果:在恶性细胞中没有MPE样本具有阳性的程序性死亡-配体1表达,尽管11种PT中有3种程序性死亡配体1表达阳性(在恶性细胞中表达超过1%)。总的来说,在LADC样本中,分化簇3(CD3)T细胞和CD3CD8细胞毒性T细胞占主导地位(MPE与PT的中位数百分比:45.6%对40.7%和4.7%对6.6%,分别)与BC相比。CD68+巨噬细胞在BC样品中占主导地位(MPE61.2%对PT57.1%),但在LADC样品中没有。一般来说,在PT中,CD3+CD8+叉头盒P3+T细胞和从恶性细胞到CD3+CD8+Ki67+和CD3+程序性细胞死亡蛋白1+T细胞的中值距离与PT诊断后早期MPE相关。
结论:MPE和PT的免疫细胞表型在每种癌症类型中相似,但BC与LADC之间不同。MPE分析可以潜在地用作PT分析的替代品,但是对这个主题的扩大研究是必不可少的。