关键词: Adequacy biopsies immunofluorescence kidney light microscopy smartphone

来  源:   DOI:10.4103/ijn.ijn_323_23   PDF(Pubmed)

Abstract:
UNASSIGNED: The utilization of smartphone-assisted evaluation is emerging in the field of histopathology. This technique improves the adequacy of samples at the bedside, avoids procedure-related complications, reduces unnecessary repeat biopsies, and saves the cost of the procedure. This study aims to compare the number of glomeruli in a renal biopsy specimen obtained by an ultrasound-guided percutaneous needle biopsy, counted at the bedside using a smartphone fitted with a 16-megapixel macro lens (Bedside method) with that observed under a light microscope after the processing of the biopsy specimen (LM method).
UNASSIGNED: In this prospective cohort study, 24 consecutive adult patients (48 kidney biopsy samples) who underwent kidney biopsies were enrolled. All specimens were extracted by an ultrasound-guided percutaneous renal biopsy from the lower pole of the left kidney. Patients\' demographics and clinical data were prospectively collected. The number of glomeruli in all the biopsy specimens was counted using a smartphone fitted with a 16-megapixel macro lens at the bedside (Bedside method) and subsequently under a light microscope by a pathologist after processing the biopsy specimen (LM method). Seven or more glomeruli in the specimen were considered adequate in our study.
UNASSIGNED: The mean age of patients at biopsy was 46.9 ± 16 years with slightly male predominance (54.2%). A total of 47 specimens were obtained from 24 patients. Of the 24 patients, 22 had native kidney biopsy and 2 had renal allograft biopsy. The average number of cores obtained per patient was 1.96. The length of core specimens ranged from 1.5 to 2 cm. A good agreement was found between bedside adequacy and slide adequacy, κ =0.684, P = 0.000. The positive agreement rate and negative agreement rate were 91.4% and 23.1%, respectively.
UNASSIGNED: In the modern era of technology, the smartphone is a good tool to evaluate the adequacy of biopsy specimens at the bedside.
摘要:
在组织病理学领域,智能手机辅助评估的应用正在兴起。这种技术提高了床边样品的充足性,避免与手术相关的并发症,减少不必要的重复活检,并节省了程序的成本。这项研究旨在比较通过超声引导经皮穿刺活检获得的肾活检标本中的肾小球数量,使用装有16百万像素微距镜头的智能手机在床边计数(床边方法),在活检标本处理后在光学显微镜下观察(LM方法)。
在这项前瞻性队列研究中,纳入24例接受肾脏活检的连续成人患者(48例肾脏活检样本)。所有标本均通过超声引导下经皮肾活检从左肾下极提取。前瞻性收集患者的人口统计学和临床数据。所有活检标本中的肾小球数量使用在床边装有16兆像素微距透镜的智能手机(床边方法)进行计数,然后在处理活检标本后由病理学家在光学显微镜下进行计数(LM方法)。在我们的研究中,标本中的七个或更多肾小球被认为是足够的。
活检患者的平均年龄为46.9±16岁,男性占主导地位(54.2%)。共从24名患者中获得47个标本。在24名患者中,22例进行了天然肾活检,2例进行了同种异体肾移植活检。每位患者获得的平均核数为1.96。岩心标本的长度范围为1.5至2厘米。床边充分性和幻灯片充分性之间有很好的一致性,κ=0.684,P=0.000。正协议率和负协议率分别为91.4%和23.1%,分别。
在现代技术时代,智能手机是评估床边活检标本是否充足的好工具。
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