内皮细胞和上皮细胞在形态上不同,在白色念珠菌感染期间在宿主防御中起关键作用。两种细胞都通过激活各种信号通路和基因表达模式来响应白色念珠菌感染。它们与这些病原体的相互作用会产生有益和有害的影响,对这些相互作用的更好理解可以帮助指导白色念珠菌感染的新疗法的开发。为了确定白色念珠菌感染期间人内皮细胞和口腔上皮细胞转录组学的差异和相似性,我们通过将共有模块与内皮特异性模块相关联并分析相关基因,对32个RNA-seq样本进行了共有WGCNA.该分析导致鉴定了14个不同的模块。我们证明了品红模块与每个数据集中的白色念珠菌感染显著相关。此外,我们发现两个数据集中的蓝色和青色模块与白色念珠菌感染具有相反的相关系数。然而,两个数据集之间的相关系数和p值略有不同.来自内皮细胞的基因中心的功能分析阐明了TNF的富集,年龄-愤怒,MAPK,和NF-κB信号。另一方面,糖酵解,丙酮酸代谢,氨基酸,果糖,甘露糖,和维生素B6代谢富集在上皮细胞中。然而,线粒体自噬,坏死,凋亡过程,缺氧在内皮细胞和上皮细胞中均富集。使用STRING和CytoHubba进行的蛋白质-蛋白质相互作用分析揭示了STAT3,SNRPE,BIRC2和NFKB2作为内皮hub基因,而RRS1,SURF6,HK2和LDHA基因在上皮细胞中被鉴定。了解这些异同可能为白色念珠菌感染的发病机制以及新的治疗靶点和干预策略的发展提供新的见解。
Endothelial and epithelial cells are morphologically different and play a critical role in host defense during Candida albicans infection. Both cells respond to C. albicans infection by activating various signaling pathways and gene expression patterns. Their interactions with these pathogens can have beneficial and detrimental effects, and a better understanding of these interactions can help guide the development of new therapies for C. albicans infection. To identify the differences and similarities between human endothelial and oral epithelial cell transcriptomics during C. albicans infection, we performed
consensus WGCNA on 32 RNA-seq samples by relating the
consensus modules to endothelial-specific modules and analyzing the genes connected. This analysis resulted in the identification of 14 distinct modules. We demonstrated that the magenta module correlates significantly with C. albicans infection in each dataset. In addition, we found that the blue and cyan modules in the two datasets had opposite correlation coefficients with a C. albicans infection. However, the correlation coefficients and p-values between the two datasets were slightly different. Functional analyses of the hub of genes from endothelial cells elucidated the enrichment in TNF, AGE-RAGE, MAPK, and NF-κB signaling. On the other hand, glycolysis, pyruvate metabolism, amino acid, fructose, mannose, and vitamin B6 metabolism were enriched in epithelial cells. However, mitophagy, necroptosis, apoptotic processes, and hypoxia were enriched in both endothelial and epithelial cells. Protein-protein interaction analysis using STRING and CytoHubba revealed STAT3, SNRPE, BIRC2, and NFKB2 as endothelial hub genes, while RRS1, SURF6, HK2, and LDHA genes were identified in epithelial cells. Understanding these similarities and differences may provide new insights into the pathogenesis of C. albicans infections and the development of new therapeutic targets and interventional strategies.