嗜盐菌是在极端高盐环境中茁壮成长的生物。尽管对它们的生物技术潜力进行了广泛的研究,嗜盐原核生物合成纳米颗粒的能力仍未得到充分研究。在这项研究中,研究了来自太阳盐池的古细菌和细菌嗜盐菌,用于银和硒纳米颗粒的细胞内/细胞外合成。银纳米颗粒是由古细菌Haloferaxsp。(AgNP-A,细胞内)和细菌Halomonassp。(AgNP-B,胞外),而细胞内硒纳米颗粒是由古细菌卤素生物产生的。(SeNP-A)和细菌芽孢杆菌。(SNP-B)。纳米粒子通过各种技术进行表征,包括紫外-可见光谱,XRD,DLS,ICP-OES,Zeta电位,FTIR,EDX,SEM,和TEM。基于TEM分析,AgNP-A和AgNP-B的平均粒度为26.34nm和22nm。此外,在XRD分析中观察到晶畴尺寸为13.01nm和6.13nm的面心立方的特征布拉格峰,分别。根据TEM分析,SeNP-A和SeNP-B菌株的晶体学表征显示出六方微晶结构,域尺寸为30.63nm和29.48nm,平均尺寸为111.6nm和141.6nm。分别。AgNP-A的多分散指数,AgNP-B,SeNP-A,和SeNP-B被确定为0.26、0.28、0.27和0.36,并且显示出纳米颗粒的高均匀性。所有合成的纳米颗粒都是稳定的,它们的ζ电位计算为(mV):对于AgNP-A,-33.12,-35.9,-31.2和-29.34,AgNP-B,SeNP-A,和SNP-B,分别。纳米颗粒对各种细菌病原体显示出抗菌活性。这项研究的结果表明,(极端)嗜盐的原核生物在纳米颗粒的绿色合成方面具有巨大的潜力。
Halophiles are the organisms that thrive in extreme high salt environments. Despite the extensive studies on their biotechnological potentials, the ability of halophilic prokaryotes for the synthesis of nanoparticles has remained understudied. In this study, the archaeal and bacterial halophiles from a solar saltern were investigated for the intracellular/extracellular synthesis of silver and selenium nanoparticles. Silver nanoparticles were produced by the archaeal Haloferax sp. (AgNP-A, intracellular) and the bacterial Halomonas sp. (AgNP-B, extracellular), while the intracellular selenium nanoparticles were produced by the archaeal Halogeometricum sp. (SeNP-A) and the bacterial Bacillus sp. (SeNP-B). The nanoparticles were characterized by various techniques including UV-Vis spectroscopy, XRD, DLS, ICP-OES, Zeta potentials, FTIR, EDX, SEM, and TEM. The average particle size of AgNP-A and AgNP-B was 26.34 nm and 22 nm based on TEM analysis. Also, the characteristic Bragg peaks of face-centered cubic with crystallite domain sizes of 13.01 nm and 6.13 nm were observed in XRD analysis, respectively. Crystallographic characterization of SeNP-A and SeNP-B strains showed a hexagonal crystallite structure with domain sizes of 30.63 nm and 29.48 nm and average sizes of 111.6 nm and 141.6 nm according to TEM analysis, respectively. The polydispersity index of AgNP-A, AgNP-B, SeNP-A, and SeNP-B was determined as 0.26, 0.28, 0.27, and 0.36 and revealed high uniformity of the nanoparticles. All of the synthesized nanoparticles were stable and their zeta potentials were calculated as (mV): -33.12, -35.9, -31.2, and -29.34 for AgNP-A, AgNP-B, SeNP-A, and SeNP-B, respectively. The nanoparticles showed the antibacterial activity against various bacterial pathogens. The results of this study suggested that the (extremely) halophilic prokaryotes have great potentials for the green synthesis of nanoparticles.