Abstract：
To determine the genotypes of the epidemic strains of Echinococcus granulosus in livestock in Tibet, samples of E. granulosus cysts were collected from 11 yaks and 62 sheep. Genomic DNA was extracted from these samples, and gene fragments of mitochondrial cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit I (nad1) were amplified by PCR and sequenced. DNASTAR and MAGA7.0 were employed for homology analysis and phylogenetic tree construction. Echinococcus granulosus cysts were detected in 56.2% (41/73) of the samples screened. Of these, 63.4% (26/41) were identified as E. granulosus G1 genotype (common sheep strain), 24.4% (10 /41) as G3 genotype (buffalo strain), and 12.2% (5/41) were G6 genotype (camel strain). The study concludes that yaks and sheep in Langkazi county, Tibet, carry three E. granulosus genotypes (G1, G3, and G6), with the G1 genotype the predominant genotype in the region. This study clarifies the distribution of E. granulosus genotypes, providing genetic data and insight for the surveillance and prevention of echinococcosis.
摘要：
为确定西藏家畜细粒棘球蚴流行株的基因型,收集了11只牦牛和62只绵羊的细粒大肠杆菌囊肿样本。从这些样本中提取基因组DNA,通过PCR扩增线粒体细胞色素c氧化酶亚基I（cox1）和NADH脱氢酶亚基I（nad1）的基因片段并进行测序。DNASTAR和MAGA7.0用于同源性分析和系统发育树构建。56.2%(41/73)的标本检出细粒棘球蚴囊肿。其中,63.4%(26/41)被鉴定为细粒大肠杆菌G1基因型(普通绵羊株)，24.4%(10/41)为G3基因型(水牛株)，G6基因型(骆驼株)占12.2%(5/41)。研究得出的结论是，浪卡子县的牦牛和绵羊，西藏,携带三种细粒大肠杆菌基因型(G1、G3和G6)，G1基因型是该地区的主要基因型。本研究阐明了细粒大肠杆菌基因型的分布，为包虫病的监测和预防提供遗传数据和见解。
