背景:术语“WORKbiota”已用于描述职业暴露和工作类型对人类微生物群组成的影响。航空公司飞行员,建筑工人,健身教练包括三个不同的专业团体,每个人都有不同的工作环境和生活方式因素,这些因素可能会显著影响他们的肠道。
目的:当前的初步调查旨在比较航空公司飞行员中特定肠道微生物的相对丰度,建筑工人,和健身教练阐明任何重大差异。通过仔细检查这些不同的专业团体,我们的目的是提高我们对职业因素如何影响肠道微生物群的认识,同时确定职业医学可能的影响.
方法:由60名代表三个不同专业领域的男性组成的便利样本-航空公司飞行员,建筑工人,和健身教练(每组20人)-在定期门诊职业健康咨询中被选中。选择的肠道微生物群成分的丰度,包括大肠杆菌,Smithii甲烷棒杆菌,Akkermansiamuciniphila,普氏粪杆菌,乳杆菌属。,双歧杆菌属。,和拟杆菌属。,使用定量SYBRGreen定量实时聚合酶链反应(qRT-PCR)对粪便样品进行定量。
结果:关于大肠杆菌的组之间没有显著差异,Smithii甲烷棒杆菌,双歧杆菌属。,和拟杆菌属。然而,乳杆菌属。与航空公司飞行员和建筑工人相比,健身教练的微生物群中的prausnitzii和Faecaliacteriumprausnitzii明显更丰富,后两组之间没有显着差异。值得注意的是,大量的Akkermansiamuciniphila表明,从健身教练到建筑工人,最终到航空公司飞行员,表现出最低水平。
结论:航空公司飞行员的肠道微生物群的特征是促进健康的细菌种类较少,包括乳酸菌。,普氏粪杆菌,和阿克曼西亚粘液虫。未来的研究对于确定是否有针对性的干预措施至关重要,例如益生菌和益生元补充剂,可能会增强特定职业群体的肠道微生物群组成和整体健康。
BACKGROUND: The term \"WORKbiota\" has been used to describe the impact of occupational exposure and work types on human microbiota composition. Airline pilots, construction workers, and fitness instructors encompass three diverse professional groups, each with distinct work environments and lifestyle factors that may significantly influence their intestinal \"WORKbiota.\"
OBJECTIVE: The current preliminary investigation was aimed to compare the relative abundance of specific gut microbes among airline pilots, construction workers, and fitness instructors to shed light on any significant differences. By scrutinizing these diverse professional groups, our objective was to enhance our understanding of how occupational factors influence gut microbiota while identifying possible implications for occupational medicine.
METHODS: A convenience sample consisting of 60 men representing three different professional domains - airline pilots, construction workers, and fitness instructors (with 20 individuals in each group) - was selected during regular outpatient occupational health consultations. The abundance of selected gut microbiota constituents, including Escherichia coli, Methanobrevibacter smithii, Akkermansia muciniphila, Faecalibacterium prausnitzii, Lactobacillus spp., Bifidobacterium spp., and Bacteroides spp., was quantified using quantitative SYBR Green quantitative real-time polymerase chain reaction (qRT-PCR) in stool samples.
RESULTS: There were no significant variations among the groups concerning Escherichia coli, Methanobrevibacter smithii, Bifidobacterium spp., and Bacteroides spp. However, Lactobacillus spp. and Faecalibacterium prausnitzii were significantly more abundant in the microbiota of fitness instructors compared to both airline pilots and construction workers, with no significant differences observed between the latter two groups. Notably, the abundance of Akkermansia muciniphila demonstrated a progressive decline from fitness instructors to construction workers and ultimately to airline pilots, who exhibited the lowest levels.
CONCLUSIONS: Airline pilots\' gut microbiota was characterized by a lower abundance of health-promoting bacterial species, including Lactobacillus spp., Faecalibacterium prausnitzii, and Akkermansia muciniphila. Future research is essential to determine whether targeted interventions, such as probiotic and prebiotic supplementation, could potentially enhance gut microbiota composition and overall health in particular occupational groups.