癌症改变了细胞的结构完整性和形态。因此,细胞功能被遮蔽。在这项研究中,对微量移液管抽吸过程进行计算建模,以预测结直肠癌细胞的力学行为。预期的癌细胞被建模为不可压缩的新Hookean粘性超弹性材料。此外,假定微量移液管是刚性的,没有变形。通过体外研究验证了所提出的模型。为了捕捉细胞的平衡和时间依赖性行为,斜坡,和蠕变试验分别采用有限元方法进行。通过模拟,研究了微量移液管几何形状和抽吸压力对结直肠癌细胞系的影响。我们的研究结果表明,随着微量移液器内半径的增加,尽管变形率和吸气长度增加,达到平衡状态的时间增加。然而,很明显,增加尖端曲率半径对吸气长度的变化影响很小。但是,由于应力集中的减少,它大大减少了平衡时间,并显着增加了变形率。有趣的是,我们的结果表明,增加抽吸压力以某种方式导致细胞变硬,从而降低变形率上升的趋势,平衡时间,和吸气长度。我们的发现为细胞治疗和癌症治疗的研究人员提供了有价值的见解,并有助于开发更精确的微流体。
Cancer alters the structural integrity and morphology of cells. Consequently, the cell function is overshadowed. In this
study, the micropipette aspiration process is computationally modeled to predict the mechanical behavior of the colorectal cancer cells. The intended cancer cells are modeled as an incompressible Neo-Hookean visco-hyperelastic material. Also, the micropipette is assumed to be rigid with no deformation. The proposed model is validated with an in-vitro
study. To capture the equilibrium and time-dependent behaviors of cells, ramp, and creep tests are respectively performed using the finite element method. Through the simulations, the effects of the micropipette geometry and the aspiration pressure on the colorectal cancer cell lines are investigated. Our findings indicate that, as the inner radius of the micropipette increases, despite the increase in deformation rate and aspirated length, the time to reach the equilibrium state increases. Nevertheless, it is obvious that increasing the tip curvature radius has a small effect on the change of the aspirated length. But, due to the decrease in the stress concentration, it drastically reduces the equilibrium time and increases the deformation rate significantly. Interestingly, our results demonstrate that increasing the aspiration pressure somehow causes the cell stiffening, thereby reducing the upward trend of deformation rate, equilibrium time, and aspirated length. Our findings provide valuable insights for researchers in cell therapy and cancer treatment and can aid in developing more precise microfluidic.