背景和目的:本研究综述了脂肪组织来源的可注射治疗骨关节炎(OA)动物模型的最新趋势,专注于最低限度地操纵或轻度处理的脂肪组织。通过评估和检查在不同的动物OA模型中研究这些疗法的特定背景,这篇综述旨在提供有价值的见解,这些见解将为未来的研究和临床应用提供信息和指导,以持续寻求骨关节炎的有效治疗方法。材料和方法:本研究对PubMed和Embase进行了全面的文献综述,以确定使用动物模型研究的骨关节炎的最小操作脂肪组织来源的可注射疗法的研究。主要搜索找到530个结果。在排除关注自发性骨关节炎的文章后;在转染时,预处理,培养,或共培养的脂肪干细胞;以及全文不可用的文章,我们在评论中包含了11篇文章。结果:所检查的疗法包括通过胶原酶消化和离心获得的机械微破碎脂肪组织(MFAT)和基质血管部分(SVF)。这些干预措施在各种动物模型中进行了评估,包括老鼠,老鼠,兔子,和诱发OA的绵羊。值得注意的是,更多的研究集中在手术诱导的OA,而不是化学诱导的OA。对这些疗法的评估集中在阐明其保护性免疫调节,抗炎,和软骨再生潜力通过综合评估,包括宏观评估,组织学分析,免疫组织化学检查,和生化化验。结论:这篇综述提供了对不同动物模型中脂肪组织来源的骨关节炎可注射疗法的综合分析。在揭示潜在好处和见解的同时,数据的异质性和研究数量有限,突出表明需要进一步研究,为临床应用制定结论性建议.
Background and Objectives: This scoping
review investigates recent trends in adipose tissue-derived injectable therapies for osteoarthritis (OA) in animal models, focusing on minimally manipulated or lightly processed adipose tissue. By evaluating and examining the specific context in which these therapies were investigated across diverse animal OA models, this
review aims to provide valuable insights that will inform and guide future research and clinical applications in the ongoing pursuit of effective treatments for osteoarthritis. Materials and Methods: This research conducted a comprehensive literature
review of PubMed and Embase to determine studies about minimally manipulated adipose tissue-derived injectable therapies for osteoarthritis investigated using animal models. The primary search found 530 results. After excluding articles that focused on spontaneous osteoarthritis; on transfected, preconditioned, cultured, or co-cultured adipose-derived stem cells; and articles with unavailable full text, we included 11 articles in our
review. Results: The examined therapies encompassed mechanical micro-fragmented adipose tissue (MFAT) and stromal vascular fraction (SVF) obtained via collagenase digestion and centrifugation. These interventions were evaluated across various animal models, including mice, rats, rabbits, and sheep with induced OA. Notably, more studies concentrated on surgically induced OA rather than chemically induced OA. The assessment of these therapies focused on elucidating their protective immunomodulatory, anti-inflammatory, and chondroregenerative potential through comprehensive evaluations, including macroscopic assessments, histological analyses, immunohistochemical examinations, and biochemical assays. Conclusions: This
review provides a comprehensive analysis of adipose tissue-derived injectable therapies for osteoarthritis across diverse animal models. While revealing potential benefits and insights, the heterogeneity of data and the limited number of studies highlight the need for further research to formulate conclusive recommendations for clinical applications.