目的:顺铂(CP)是一种广泛使用的化疗药物,但其严重的副作用影响睾丸功能。我们研究了越橘提取物对CP诱导的睾丸毒性的潜在保护作用。
方法:成年雄性白化大鼠40只,分为4组。对照动物接受单一口服剂量的0.9%盐水。越橘处理的大鼠每天接受口服越橘提取物(200mg/kg体重[BW]溶解在1mL盐水中),连续10天。向CP处理的动物施用单次腹膜内剂量(7.5mg/kgBW)。最后,越橘+CP组连续10天每天口服越橘提取物(200mg/kgBW),在第2天使用一次腹膜内剂量的CP(7.5mg/kgBW)。我们评估了精子数量,运动性,生存能力,和异常,随着睾丸的重量,睾丸重量与体重之比,抗氧化活性,氧化应激标志物水平(丙二醛[MDA]和过氧化氢[H2O2]),性激素(促卵泡激素[FSH],促黄体激素[LH],和睾丸激素),以及凋亡和抗凋亡标志物,和DNA损伤。睾丸组织进行组织病理学检查。
结果:在CP治疗的大鼠中,睾丸重量;睾丸重量与体重之比;FSH水平,LH,睾丸激素,超氧化物歧化酶,过氧化氢酶,谷胱甘肽S-转移酶,谷胱甘肽,和B细胞淋巴瘤2;和精子计数,运动性,和正常精子的比例。CP给药与高MDA有关,H2O2,p53,Bax,细胞色素c,caspase9和caspase3水平,随着提升的尾部力矩。然而,越橘提取物给药显着改善了所有改变的参数。
结论:越橘治疗具有保护作用,并通过抗氧化活性和细胞保护作用降低了CP诱导的睾丸毒性。
OBJECTIVE: Cisplatin (CP) is a widely used chemotherapeutic agent, but its severe side effects impact testicular function. We investigated the potential protective effects of bilberry extract against CP-induced testicular toxicity.
METHODS: Forty adult male albino rats were divided into four groups. Control animals received a single oral dose of 0.9% saline. Bilberry-treated rats received oral bilberry extract (200 mg/kg body weight [BW] dissolved in 1 mL of saline) daily for 10 consecutive days. CP-treated animals were administered a single intraperitoneal dose (7.5 mg/kg BW). Finally, a bilberry+CP group received oral bilberry extract (200 mg/kg BW) daily for 10 consecutive days, with one intraperitoneal dose of CP (7.5 mg/kg BW) on day 2. We assessed sperm count, motility, viability, and abnormalities, along with testis weight, testis weight-to-BW ratio, antioxidant activity, levels of oxidative stress markers (malondialdehyde [MDA] and hydrogen peroxide [H2O2]), sex hormones (follicle-stimulating hormone [FSH], luteinizing hormone [LH], and testosterone), and apoptotic and anti-apoptotic markers, and DNA damage. Testicular tissue underwent histopathological examination.
RESULTS: Among CP-treated rats, significantly lower values were observed for testis weight; testis weight-to-BW ratio; levels of FSH, LH, testosterone, superoxide dismutase, catalase, glutathione S-transferase, glutathione, and B-cell lymphoma 2; and sperm count, motility, and proportion of normal sperm. CP administration was associated with higher MDA, H2O2, p53, Bax, cytochrome c, caspase 9, and caspase 3 levels, along with elevated tail moment. However, bilberry extract administration significantly improved all altered parameters.
CONCLUSIONS: Bilberry treatment demonstrated protective effects and reduced CP-induced testicular toxicity via antioxidant activity and cytoprotection.