吸气剂,热带海草,因其对海洋生态系统的重大贡献以及生物活性化合物的潜在健康益处而闻名。本研究旨在比较通过超声辅助提取(UAE)和微波辅助提取(MAE)使用绿色提取的类胡萝卜素水平,并评估这些提取物对抗氧化应激的生物学特性。糖尿病,通过计算机模拟和体外分析和肥胖。E.acoroides样本是从万老市收集的,印度尼西亚,并受到阿联酋和MAE的影响。使用UHPLC-ESI-MS/MS分析提取物以鉴定类胡萝卜素,包括β-胡萝卜素,叶黄素,番茄红素,β-隐黄质,和玉米黄质.进行了计算机模拟分析以预测化合物的生物活性,毒性,使用WAY2DRUGPASS和与CB-Dock2的分子对接。化合物C3、C4和C7表现出显著的相互作用,关键的代谢蛋白和microRNAs,进一步验证其潜在的治疗益处。体外测定使用DPPH和FRAP测定评估抗氧化活性,通过抑制α-葡萄糖苷酶和α-淀粉酶,通过脂肪酶抑制和MTT法检测3T3-L1细胞的抗肥胖作用。结果表明,UAE和MAE提取物均表现出显着的抗氧化剂,抗糖尿病药,和抗肥胖活动。与UAE提取物相比,MAE提取物显示出更高的类胡萝卜素含量和更大的生物活性。这些发现表明acoroides,主要是在使用MAE提取时,具有作为开发海洋抗氧化剂的天然生物活性化合物来源的潜力,抗糖尿病药,和抗肥胖药。本研究通过提供对E.acoroides类胡萝卜素的有效提取方法和治疗潜力的见解来补充现有文献。
Enhalus acoroides, a tropical seagrass, is known for its significant contribution to marine ecosystems and its potential health benefits due to bioactive compounds. This
study aims to compare the carotenoid levels in E. acoroides using green extraction via ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) and to evaluate the biological properties of these extracts against oxidative stress, diabetes, and obesity through in silico and in vitro analyses. E. acoroides samples were collected from Manado City, Indonesia, and subjected to UAE and MAE. The extracts were analyzed using UHPLC-ESI-MS/MS to identify carotenoids, including β-carotene, lutein, lycopene, β-cryptoxanthin, and zeaxanthin. In silico analysis was conducted to predict the compounds\' bioactivity, toxicity, and drug-likeness using WAY2DRUG PASS and molecular docking with CB-Dock2. The compounds C3, C4, and C7 demonstrated notable interactions, with key metabolic proteins and microRNAs, further validating their potential therapeutic benefits. In vitro assays evaluated antioxidant activities using DPPH and FRAP assays, antidiabetic properties through α-glucosidase and α-amylase inhibition, and antiobesity effects via lipase inhibition and MTT assay with 3T3-L1 cells. Results indicated that both UAE and MAE extracts exhibited significant antioxidant, antidiabetic, and antiobesity activities. MAE extracts showed higher carotenoid content and greater biological activity compared to UAE extracts. These findings suggest that E. acoroides, mainly when extracted using MAE, has promising potential as a source of natural bioactive compounds for developing marine-based antioxidant, antidiabetic, and antiobesity agents. This
study supplements existing literature by providing insights into the efficient extraction methods and the therapeutic potential of E. acoroides carotenoids.