Nuclear power plants, recognized for their extended operational life, minimal greenhouse gas emissions, and high-power density, are deemed as reliable energy sources. Nonetheless, concerns persist regarding the radioactive discharges from these plants and their potential impact on health and the environment. To comprehend the radiological implications of such releases, this study presents, for the first time, an analysis of radiological data from 7 Indian nuclear power plants (NPPs), collected by Indian environmental survey laboratories (ESL) over the past two decades (2000-2020). This dataset encompasses radioactivity concentrations in the atmospheric, aquatic, and terrestrial environments within a 30 km radius of each NPP, as well as the annual cumulative external gamma doses recorded by environmental thermoluminescence dosimeters (TLDs). The analysis yielded several key findings: (i) Radioactivity concentrations around the NPPs were low and comparable to values measured at other nuclear power plant sites worldwide; (ii) Tritium concentrations in receiving water bodies were <1 % of the internationally recommended limit of 10,000 Bq/l; (iii) The estimated total radiation doses to the public were at most 10 % of the stipulated regulatory dose limit of 1000 μSv and consistently decreased over the study period and (iv) Variations in doses among the NPP sites were primarily attributed to legacy technology used in specific reactors. These results indicate efficient and secure reactor operations and the minimal contribution of Indian nuclear power plants to anthropogenic doses in the country. The findings hold potential significance for reinforcing India\'s commitment to advancing its nuclear power program.

Strongylocentrotus nudus egg polysaccharide (SEP) extracted from sea urchins has potential anticancer activity. However, little is known about its pharmacokinetic properties. To investigate the pharmacokinetics of SEP, it was radiolabeled with tritium. Furthermore, a sensitive, selective, and rapid liquid scintillation counter (LSC) method for quantifying 3H-SEP in biological matrix was validated. The lower quantification limit of the method was 4 Bq. The relative standard deviations (RSDs) of the intra- and inter-day precision were <3.0% and <3.9%, respectively. 3H-SEP was successfully applied to investigate the pharmacokinetics of SEP after intravenous administration of 20, 40, and 80 mg/kg (40 μCi/kg) in rats and 5, 10, and 20 mg/kg (6 μCi/kg) in beagles. The AUC(0-t) of SEP at three different doses was 487.81 ± 39.99 mg/L*h, 1,003.10 ± 95.94 mg/L*h, and 2,188.84 ± 137.73 mg/L*h in rats and 144.12 ± 3.78 mg/L*h, 322.62 ± 28.03 mg/L*h, and 754.17 ± 37.79 mg/L*h in beagles. The terminal elimination half-life (t1/2) of SEP was longer in beagles (204.29 ± 139.34 h) than in rats (35.48 ± 6.04 h). The concentration of SEP in plasma declined rapidly in both rats and beagles. All the study results provide detailed pharmacokinetic profiles of SEP in two kinds of animals, which will be helpful for further development.

Tritium, whether naturally occurring or caused by human nuclear activity, can result in a large amount of tritium contamination in the environment, especially in the water cycle, causing a high concentration of tritium in rainfall. The objective of this research was to measure the level of tritium in the environment from rainfall in two different areas as a basis for monitoring tritium contamination in the environment. Rainwater samples were collected in Thailand every 24 h for a period of 1 year during 2021-2022 at the Kasetsart University Station, Sriracha Campus, Chonburi province and at the Mae Hia Agricultural Meteorological Station, Chiang Mai province. The tritium levels were measured in rainwater samples using the electrolytic enrichment method combined with liquid scintillation counting. The chemical composition of the rainwater was analyzed based on ion chromatography. The results (presented with ± combined uncertainty) showed that the tritium content in the rainwater samples at Kasetsart University Station Sriracha Campus was in the range 0.9 ± 0.2-1.6 ± 0.3 TU (0.11 ± 0.02-0.19 ± 0.03 Bq.L-1). The mean concentration was 1.0 ± 0.2 TU (0.12 ± 0.03 Bq.L-1). The most common ions found in the rainwater samples were SO42-, Ca2+, and NO3-, with mean concentrations of 1.52 ± 0.82, 1.08 ± 0.51, and 1.05 ± 0.78 mg.L-1, respectively. The tritium content in rainwater collected from the Mae Hia Agricultural Meteorological Station was in the range 1.6 ± 0.2-4.9 ± 0.4 TU (0.19 ± 0.02-0.58 ± 0.05 Bq.L-1). The mean concentration was 2.4 ± 0.4 TU (0.28 ± 0.05 Bq.L-1). The most common ions found in the rainwater were NO3-, Ca2+, and SO42-, with mean concentrations of 1.21 ± 1.02, 0.67 ± 0.43, and 0.54 ± 0.41 mg.L-1, respectively. The tritium concentration in the rainwater at both stations differed but remained at a natural level (less than 10 TU). There was no correlation between the tritium concentration and the chemical composition of the rainwater. The tritium levels obtained from this study could be used as a basis for reference and monitoring of future environmental changes due to nuclear accidents or activities, both domestically and internationally.

Currently, international nuclear fission reactors producing medical isotopes face the problem of shutdown and maintenance, decommissioning, or dismantling, while the production capacity of domestic research reactors for medical radioisotopes is inadequate, and the supply capacity for medical radioisotopes faces major challenges in the future. Fusion reactors are characterized by high neutron energy, high flux density, and the absence of highly radioactive fission fragments. Additionally, compared to fission reactors, the reactivity of the fusion reactor core is not significantly affected by the target material. By building a preliminary model of the China Fusion Engineering Test Reactor (CFETR), a Monte Carlo simulation was performed for particle transport between different target materials at a fusion power of 2 GW. The yields (specific activity) of six medical radioisotopes (14C, 89Sr, 32P, 64Cu, 67Cu, and 99Mo) with various irradiation positions, different target materials, and different irradiation times were studied, and compared with those of other high-flux engineering test reactors (HFETR) and the China Experimental Fast Reactor (CEFR). The results show that this approach not only provides competitive medical isotope yield, but also contributes to the performance of the fusion reactor itself, e.g., tritium self-sustainability and shielding performance.

The mutation, apoptosis and chromosomal aberration induced by tritiated water (HTO) in spleen T lymphocytes of mice were investigated and compared with those by acute or chronic 137Cs gamma irradiation. p53 wild-type (p53+/+) and null (p53-/-) mice were exposed to tritium (3H) beta rays via a single injection of HTO. 137Cs gamma irradiation was carried out at dose-rate of 0.86Gy min-1 (acute) and at a low dose-rate (0.71-0.09 mGy min-1) that mimicked internal exposure (gamma simulation-irradiation). Each dose of irradiation was 3Gy. When compared on the basis of the induced TCR variant fractions in p53-/- mice at 3Gy, 3H beta rays appeared to be more mutagenic than chronic gamma ray reference. On the other hand, both of the frequency chromosomal aberration was not different significantly between HTO injected and 137Cs gamma irradiated mice.

The Christchurch groundwater system is an exceptional water resource with very high drinking water quality supplying all the water requirements of the city. The groundwater system has changed over the years because of rising groundwater abstraction due to increasing population and development. The present (2017) data revealed slightly older 14C ages and increasingly steep west-east age gradients compared to the earlier work from 1976 to 2006, showing continued upflow of deep water into the exploited aquifers which is much older on the east (coastal) side than on the west (inland) side. In addition, the 3H ages for wells on the west side of the system are often much younger than their 14C ages showing that there is input of young shallow water to the wells in addition to the deep water input. Application of a binary model identifies the ages and amounts of the two components, showing that the young component is becoming younger although smaller as a proportion of the flow, and the old component from depth is becoming larger. Newly completed wells near the Waimakariri River have allowed identification of the young component, which is almost entirely composed of very young Waimakariri River seepage at all depths and therefore has very little chemical loading. Instead any chemical input (e.g. chloride, nitrate) to the Christchurch aquifers is being brought in by the old deep component which on the western side of the Christchurch system is derived from rainfall recharge on the developing Ashley-Waimakariri Plains area (plus river seepage). Chemical traces of this deep input from the northwest are at present very subtle, although more appreciable signals are seen in some wells further to the north of Christchurch. In the future, slowly increasing chemical input to the Christchurch aquifers on the west side of the system is to be expected as abstraction increases.

We compared the formation of γH2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing 3H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/liter. A linear dependence of changes in the number of γH2AX foci on the specific radioactivity of the medium was revealed. The quantitative yield of DNA double-strand breaks under the influence of 3H-thymidine was more than 2-fold higher than under the influence of 3H-labeled amino acids. Comparative analysis of the yields of DNA double-strand breaks during cell incubation with 3H-labeled amino acids showed that 3H-alanine produced more pronounced effect that 3H-proline, which is consistent with the data on the content of their non-radioactive analogs in chromatin proteins.

Groundwater aquifers in Morocco\'s coastal regions are under serious threat as a result of climate change. This study was conducted to evaluate and map the quality of water resources, by evaluating the level of pollution of the groundwater in the Meskala-Ouazzi sub-basin, a coastal area of Essaouira based on the physico-chemical analysis of 58 samples using a geographic information system (GIS) technique, analytical analysis, nitrate pollution index (NPI), and groundwater pollution index (GPI). The diagram piper of the study area is dominated by Cl-Ca-Mg, Cl-Na, HCO3-Ca-Mg, and SO4-Ca types. The concentrations of nitrate ranged from 2 to 175 mg/L. It was discovered that 22% of the groundwater samples had nitrate amounts greater than the World Health Organization\'s recommended maximum allowable level of 50 mg/L. The NPI ranged between - 0.9 and 7.8. According to the classification of NPI, 44.8% of the total groundwater samples represent clean water, indicating that the groundwater in the study area is suitable for irrigation. GPI values ranging from 0.6 to 3.7, with an average of 1.7, identifies 37.9% of all groundwater samples as low polluted. The inverse distance weighting (IDW) approach was used to generate a spatial distribution map, which indicates that appropriate groundwater is present in the sub-upstream basin\'s part. Overall, the forte concentration in groundwater samples detected in western and central areas showed that the nitrate originated from large amounts of nitrogen fertilizer used by humans in agricultural activities during periods of irrigation. The low tritium (δ3H) content shows that the aquifer recharge is stale water and excessive use of fertilizers leads to groundwater pollution faster over time.

UNASSIGNED: To measure medium borne bystander effects, to study the influence of radioadaptive response (RAR) on bystander response, and to discover reliable radioresponsive biomarkers in radio-adapting frogs from Duke Swamp contaminated with an above-background radiation level and in naïve frogs from Twin Lake as the background control site.
UNASSIGNED: Frogs were captured at Duke Swamp and Twin Lake and brought to the lab at the Canadian Nuclear Laboratories facility. Half of the frogs from each site were irradiated with 4 Gy while the other half of the frogs were left with no further radiation treatment. Frog bladders were removed and placed in sterile culture media. Upon arrival at McMaster University, the bladders were processed for tissue cultures. After 48 h, the culture media conditioned by the bladder explants were harvested for clonogenic reporter survival assay and calcium flux measurements for assessing bystander effects. HPV-G cells were used as bystander reporter cells in all radiation-induced bystander effect (RIBE) assays. The frog bladder cultures were incubated for another 10-12 days followed by immunochemical staining for bcl-2 and c-myc expressions to analyze cellular anti-apoptotic (pro-survival) and pro-apoptotic (pro-death) responses, respectively.
UNASSIGNED: Only culture media conditioned by bladders from 4-Gy-irradiated naïve frogs from Twin Lake induced bystander effects (reduction of HPV-G reporter cells\' clonogenic survival and presence of strong calcium flux activities). The 4 Gy irradiation dose increased pro-apoptotic c-myc expression in naïve frogs\' bladder explants. Culture media conditioned by bladders from radio-adapting frogs from Duke Swamp enhanced HPV-G\'s clonogenic survival and a 4 Gy irradiation challenge did not change the enhanced clonogenic survival nature nor induce calcium flux. In bladder explants from both control and 4-Gy-irradiated radio-adapting frogs, anti-apoptotic bcl-2 expression for pro-survival responses was ubiquitous while c-myc expression for pro-death responses was limited to a small fraction of cells.
UNASSIGNED: The clonogenic RIBE reporter assay using HPV-G and calcium flux measurements are useful diagnostic tools for RIBE assessment of field biological samples, specifically those from frogs. RAR induced by environmentally relevant low-dose radiation induces protective bystander response. Bcl-2 and c-myc are reliable biomarkers for evaluating low dose radiation responses in wild populations of amphibians. Overall, this pilot study emphasizes the importance of looking at non-targeted effects (NTEs) in natural populations of non-human biota that could be vulnerable to chronic low-dose radiation exposures.

Accurately monitoring and effectively controlling the tritium compounds based on their ro-vibrational energy structure are important issues in various nuclear systems. Because of their radioactivity, it is difficult to obtain the corresponding energies directly through experiments. In this paper, the potential energy curves and the corresponding ro-vibrational full spectrum of DT, HT and T2 systems are derived by ab initio methods. However, it is difficult to verify the reliability of the calculated results due to the lack of direct experimental support. Therefore, a data-driven reliability analysis method is proposed, which can confirm the reliability by extracting information from the relevant calculations and multiple experimental data (the vibrational level, rotational level, and molar heat capacity) of similar systems (HD, H2, D2). The results show that: 1) The potential energy curves obtained by the ab initio method can provide the full ro-vibrational energy spectrum with an accuracy of approximately 10 cm-1; 2) Macroscopic heat capacity information can be used to distinguish and calibrate the overall reliability of microscopic ro-vibrational energies; 3) For the isotopic energy level structure of hydrogen, the influence of isotopes is mainly mass effect.