背景:毛状根是由发根根瘤菌感染(以前称为发根农杆菌)产生的植物组织培养物。如今,这些根已经在生物技术中获得了更多的空间,因为它们有利于重组表达有价值的蛋白质;它包括简化的下游加工,蛋白质根际分泌,和生物反应器中的可扩展性。然而,由于报告之间的方法不一致,组织平台仍然是一项有前途的技术。
结果:在当前论文中,通过对使用烟草毛状根进行重组表达的研究的系统回顾,我们提出了克服这一问题的第一步。我们对最初选择的387种出版物中的36种进行了定性综合。按照PRISMA程序,对所有论文进行排除和纳入标准评估.探索了多个根系培养点,包括转换方法,根生长曲线,外部添加剂,并使用生物反应器扩大规模,以确定哪些方法表现最好,以及实现稳健方案仍需要什么。
结论:这里提供的信息可能有助于那些希望在实验室中研究毛状根的研究人员找到评估文献状态的成功路径。
BACKGROUND: Hairy roots are a plant-tissue culture raised by Rhizobium rhizogenes infection (formerly known as Agrobacterium rhizogenes). Nowadays, these roots have been gaining more space in biotechnology due to their benefits for the recombinant expression of valuables proteins; it includes simplified downstream processing, protein rhizosecretion, and scalability in bioreactors. However, due to methodological inconsistency among reports, the tissue platform is still a promising technology.
RESULTS: In the current paper, we propose the first step to overcome this issue through a systematic
review of studies that employ Nicotiana hairy roots for recombinant expression. We conducted a qualitative synthesis of 36 out of 387 publications initially selected. Following the PRISMA procedure, all papers were assessed for exclusion and inclusion criteria. Multiple points of root culture were explored, including transformation methods, root growth curve, external additives, and scale-up with bioreactors to determine which approaches performed best and what is still required to achieve a robust protocol.
CONCLUSIONS: The information presented here may help researchers who want to work with hairy roots in their laboratories trace a successful path to appraisal the literature status.