背景:伤口愈合过程,恢复受损组织的功能,可以通过各种化合物加速。最近的实验分析强调了植物化学物质在改善皮肤再生和伤口愈合方面的有益作用。在传统医学中,用于治疗不同损伤或皮肤疾病的广泛植物之一是GaliumaparineL.(GA)。此外,先前报道的GA化合物表明其对伤口愈合过程的治疗作用,然而,其对伤口愈合过程的细胞和分子阶段的调节作用尚未被研究。
方法:在本研究中,使用HPTLC指纹分析GA提取物的植物化学概况,并对其植物化学物质进行了进一步的科学评估。在细胞和分子水平上探索了GA提取物的伤口愈合作用,同时考虑了细胞毒性。伤口闭合增强效果,抗菌活性,和抗氧化活性进行了评估。
结果:GA提取物的HPTLC指纹图谱证明了其先前报道的植物化学特征,包括酚,黄酮类化合物,单宁,植物酸,麦角生物碱,黄酮类化合物,蒽醌,萜类化合物,固醇,水杨苷,亲脂性化合物,皂苷,环烯醚萜类,和杂环氮化合物。抗菌评估,提取物,表明金黄色葡萄球菌对GA的抑制作用比大肠杆菌和表皮葡萄球菌更敏感。DPPH测试结果揭示了GA提取物的抗氧化性能,与抗坏血酸相当。活力测定的结果表明,用不同浓度的全植物提取物处理的人脐内皮细胞(HUVEC)和正常人皮肤成纤维细胞(NHDF)细胞系没有细胞毒性作用,细胞活力以剂量依赖性方式增加。划痕测定的结果显示改善的细胞迀移和伤口闭合。
结论:这项研究表明抗氧化剂,抗微生物,GA水醇提取物的体外伤口愈合效果,这与它在传统医学中的应用相一致。未显示细胞毒性作用。这项研究的结果可以作为进一步研究的基础,如动物模型和植物化学研究。进一步评估其对伤口愈合过程中涉及的机制和信号传导途径的影响,例如血管生成和细胞增殖,可以提供对GA提取物潜在治疗效果的新见解。
BACKGROUND: The wound healing process, restoring the functionality of the damaged tissue, can be accelerated by various compounds. The recent experimental analysis highlights the beneficial effects of phytochemicals in improving skin regeneration and wound healing. In traditional medicine, one of the widespread plants used for treating different injuries or skin afflictions is Galium aparine L. (GA). Besides, previously reported chemical compounds of GA suggested its therapeutic effects for the wound healing process, yet its regulatory effects on the cellular and molecular stages of the wound healing process have not been investigated.
METHODS: In the present
study, the phytochemical profile of the GA extract was analyzed using HPTLC fingerprinting, and further scientific evaluation of its phytochemicals was done. The wound-healing effects of GA extract were explored at the cellular and molecular levels while accounting for cell toxicity. The wound closure enhancing effect, antibacterial activity, and antioxidant activity were assessed.
RESULTS: The HPTLC fingerprinting of the GA extract proved its previously reported phytochemical profile including phenols, flavonoids, tannins, plant acids, ergot alkaloids, flavonoids, anthraquinones, terpenoids, sterols, salicin, lipophilic compounds, saponins, iridoids, and heterocyclic nitrogen compounds. Antimicrobial assessment, of the extract, indicated the more susceptibility of S. aureus to the inhibitory effects of GA rather than E. coli and S. epidermidis. DPPH test results revealed the antioxidant property of GA extract, which was comparable to ascorbic acid. The results of the viability assay showed no cytotoxicity effects on human umbilical endothelial cell (HUVEC) and normal human dermal fibroblast (NHDF) cell lines treated with different concentrations of whole plant extract and cell viability increased in a dose-dependent manner. The results of the scratch assay showed improved cell migration and wound closure.
CONCLUSIONS: This
study shows the anti-oxidant, anti-microbial, and in vitro wound healing wound-healing effects of GA hydroalcoholic extract, which aligns with its use in traditional medicine. No cytotoxicity effects were shown. The results from this
study can be the basis for further investigations such as animal models and phytochemical studies. Further evaluation of its effects on mechanisms and signaling pathways involved in the wound healing processes such as angiogenesis and cell proliferation can provide novel insights into the potential therapeutic effects of the GA extract.