Abstract：
BACKGROUND: Moringa oleifera (Moringaceae family), commonly known as horseradish or tree of life, is traditionally used for various diseases, such as diabetes, hypercholesterolemia, neurological disorders, among others.
OBJECTIVE: To evaluate the toxicological profile of the oral use of an aqueous extract of Moringa oleifera leaves for 13 weeks in mice.
METHODS: Initially, a factorial design (23) was carried out to optimize aqueous extraction using as variables; the extraction method and proportion of drug. The 13-week repeated-dose toxicity trial used female and male mice, with oral administration of aqueous extract of Moringa oleifera leaves at doses of 250, 500, and 1000 mg/kg. The animals were evaluated for body weight, water and feed intake, biochemical and hematological parameters, urinalysis, ophthalmology and histopathology of the liver, spleen and kidneys.
RESULTS: The extraction efficiency was evidenced by the extraction by maceration at 5%, obtaining the optimized extract of Moringa oleifera (OEMo). The oral administration of OEMo did not promote significant difference (p > 0.05) in the weight gain, food and water consumption of the control animals and those treated with 250 and 500 mg/kg. However, treatment with 1000 mg/kg promoted a reduction (p < 0.05) in food intake and body weight from the 7th week onwards in male and female mice. No alterations were detected in the hematological and histological parameters in the concentrations tested for both sexes. The highest concentration treatment (1000 mg/kg) promoted an increase in transaminases in males and females. All concentrations promoted a significant decrease (p < 0.05) in the serum lipid profile of mice.
CONCLUSIONS: This study developed an optimized extract of Moringa oleifera leaves, which should be used with caution in preparations above 500 mg/kg for the long term because it leads to significant changes in liver enzymes. On the other hand, the extract proved to be a promising plant preparation for hyperlipidemia in mice.
OBJECTIVE: To evaluate the toxicological profile of the oral use of an aqueous extract of Moringa oleifera leaves for 13 weeks in mice.
METHODS: Initially, a factorial design (23) was carried out to optimize aqueous extraction using as variables; the extraction method and proportion of drug. The 13-week repeated-dose toxicity trial used female and male mice, with oral administration of aqueous extract of Moringa oleifera leaves at doses of 250, 500, and 1000 mg/kg. The animals were evaluated for body weight, water and feed intake, biochemical and hematological parameters, urinalysis, ophthalmology and histopathology of the liver, spleen and kidneys.
RESULTS: The extraction efficiency was evidenced by the extraction by maceration at 5%, obtaining the optimized extract of Moringa oleifera (OEMo). The oral administration of OEMo did not promote significant difference (p > 0.05) in the weight gain, food and water consumption of the control animals and those treated with 250 and 500 mg/kg. However, treatment with 1000 mg/kg promoted a reduction (p < 0.05) in food intake and body weight from the 7th week onwards in male and female mice. No alterations were detected in the hematological and histological parameters in the concentrations tested for both sexes. The highest concentration treatment (1000 mg/kg) promoted an increase in transaminases in males and females. All concentrations promoted a significant decrease (p < 0.05) in the serum lipid profile of mice.
CONCLUSIONS: This study developed an optimized extract of Moringa oleifera leaves, which should be used with caution in preparations above 500 mg/kg for the long term because it leads to significant changes in liver enzymes. On the other hand, the extract proved to be a promising plant preparation for hyperlipidemia in mice.
摘要：
背景：辣木（辣木科），通常被称为辣根或生命之树，传统上用于各种疾病，比如糖尿病，高胆固醇血症,神经系统疾病,在其他人中。
目的：评估小鼠口服使用辣木叶水提物13周的毒理学特征。
方法：最初，进行析因设计（23）以优化水提取作为变量；提取方法和药物比例。为期13周的重复剂量毒性试验使用了雌性和雄性小鼠，以250、500和1000mg/kg的剂量口服辣木叶的水提取物。评估动物的体重，水和饲料摄入量，生化和血液学参数，尿液分析,肝脏的眼科和组织病理学，脾脏和肾脏。
结果：以5%浸渍法提取，证明了提取效率，获得辣木（OEMo）的优化提取物。口服OEMo没有促进体重增加的显著差异(p>0.05),对照动物和用250和500mg/kg治疗的动物的食物和水消耗。然而,1,000mg/kg的治疗促进了从第7周开始雄性和雌性小鼠的食物摄入和体重的减少(p<0.05)。在两种性别的测试浓度中，血液学和组织学参数均未检测到变化。最高浓度处理（1000mg/kg）促进了男性和女性转氨酶的增加。所有浓度均促进小鼠血清脂质谱的显著降低(p<0.05)。
结论：本研究开发了辣木叶的优化提取物，长期在500mg/kg以上的制剂中应谨慎使用，因为它会导致肝酶的显着变化。另一方面,该提取物被证明是小鼠高脂血症的有前途的植物制剂。
目的：评估小鼠口服使用辣木叶水提物13周的毒理学特征。
方法：最初，进行析因设计（23）以优化水提取作为变量；提取方法和药物比例。为期13周的重复剂量毒性试验使用了雌性和雄性小鼠，以250、500和1000mg/kg的剂量口服辣木叶的水提取物。评估动物的体重，水和饲料摄入量，生化和血液学参数，尿液分析,肝脏的眼科和组织病理学，脾脏和肾脏。
结果：以5%浸渍法提取，证明了提取效率，获得辣木（OEMo）的优化提取物。口服OEMo没有促进体重增加的显著差异(p>0.05),对照动物和用250和500mg/kg治疗的动物的食物和水消耗。然而,1,000mg/kg的治疗促进了从第7周开始雄性和雌性小鼠的食物摄入和体重的减少(p<0.05)。在两种性别的测试浓度中，血液学和组织学参数均未检测到变化。最高浓度处理（1000mg/kg）促进了男性和女性转氨酶的增加。所有浓度均促进小鼠血清脂质谱的显著降低(p<0.05)。
结论：本研究开发了辣木叶的优化提取物，长期在500mg/kg以上的制剂中应谨慎使用，因为它会导致肝酶的显着变化。另一方面,该提取物被证明是小鼠高脂血症的有前途的植物制剂。
