PDF(Pubmed)
Abstract：
UNASSIGNED: The botanical species Bauhinia guianensis Aublet (Leguminosae-Cercidoideae) is traditionally used in the Amazon for medicinal purposes.
UNASSIGNED: The acute toxicity of the hydroethanolic extracts from B. guianensis leaves and stems (HELBg and HESBg) was evaluated in zebrafish (Danio rerio), with emphasis on the embryonic developmental stage and adult alterations.
UNASSIGNED: Extracts were analyzed on LC-DAD-MS/MS. Zebrafish eggs were inoculated individually with concentrations of HELBg and HESBg (0.25, 0.5, 0.75, 1.0, and 1.5 µg/mL), observed for 96 h. Adult zebrafish were treated with a single oral dose (100, 200, 500, 1000, and 2000 mg/kg) of HELBg and HESBg, observed for 48 h.
UNASSIGNED: HELBg and HESBg analysis detected 55 compounds. Both extracts exhibited toxicity, including embryo coagulation at higher doses of HELBg and absence of heartbeats in embryos at all doses of HESBg. Behavioral variations were observed; tissue alterations in adult zebrafish were found at the highest doses, primarily in the liver, intestine, and kidneys because of HELBg and HESBg effects. The LD50 of HESBg was 1717 mg/kg, while HELBg exceeded the limit dose of 2000 mg/kg.
UNASSIGNED: The study on acute toxicity of B. guianensis extracts exhibits significant toxic potential, emphasizing effects on embryonic and adult zebrafish. The results suggest relative safety of the species preparations, encouraging further clinical trials on potential biological activities.
UNASSIGNED: The acute toxicity of the hydroethanolic extracts from B. guianensis leaves and stems (HELBg and HESBg) was evaluated in zebrafish (Danio rerio), with emphasis on the embryonic developmental stage and adult alterations.
UNASSIGNED: Extracts were analyzed on LC-DAD-MS/MS. Zebrafish eggs were inoculated individually with concentrations of HELBg and HESBg (0.25, 0.5, 0.75, 1.0, and 1.5 µg/mL), observed for 96 h. Adult zebrafish were treated with a single oral dose (100, 200, 500, 1000, and 2000 mg/kg) of HELBg and HESBg, observed for 48 h.
UNASSIGNED: HELBg and HESBg analysis detected 55 compounds. Both extracts exhibited toxicity, including embryo coagulation at higher doses of HELBg and absence of heartbeats in embryos at all doses of HESBg. Behavioral variations were observed; tissue alterations in adult zebrafish were found at the highest doses, primarily in the liver, intestine, and kidneys because of HELBg and HESBg effects. The LD50 of HESBg was 1717 mg/kg, while HELBg exceeded the limit dose of 2000 mg/kg.
UNASSIGNED: The study on acute toxicity of B. guianensis extracts exhibits significant toxic potential, emphasizing effects on embryonic and adult zebrafish. The results suggest relative safety of the species preparations, encouraging further clinical trials on potential biological activities.
摘要：
■传统上在亚马逊地区用于药用的植物性物种紫荆花（Leguminosae-Cercidoideae）。
■在斑马鱼（Daniorerio）中评估了来自B.guianensis叶和茎（HELBg和HESBg）的乙醇提取物的急性毒性，重点是胚胎发育阶段和成人改变。
■在LC-DAD-MS/MS上分析提取物。斑马鱼卵分别接种HELBg和HESBg浓度（0.25、0.5、0.75、1.0和1.5µg/mL），观察96小时。用单次口服剂量（100、200、500、1000和2000mg/kg）的HELBg和HESBg治疗成年斑马鱼，观察48小时。
■HELBg和HESBg分析检测到55种化合物。两种提取物都表现出毒性，包括在较高剂量HERBg下的胚胎凝固和在所有剂量HESBg下的胚胎中没有心跳。观察到行为变化；在最高剂量下发现成年斑马鱼的组织改变，主要在肝脏，肠,和肾脏，因为HELBg和HESBg的影响。HESBg的LD50为1717mg/kg，而HELBg超过2000mg/kg的限制剂量。
■广西双歧杆菌提取物的急性毒性研究显示出显著的毒性潜力，强调对胚胎和成年斑马鱼的影响。结果表明该物种制剂的相对安全性，鼓励关于潜在生物活性的进一步临床试验。
■在斑马鱼（Daniorerio）中评估了来自B.guianensis叶和茎（HELBg和HESBg）的乙醇提取物的急性毒性，重点是胚胎发育阶段和成人改变。
■在LC-DAD-MS/MS上分析提取物。斑马鱼卵分别接种HELBg和HESBg浓度（0.25、0.5、0.75、1.0和1.5µg/mL），观察96小时。用单次口服剂量（100、200、500、1000和2000mg/kg）的HELBg和HESBg治疗成年斑马鱼，观察48小时。
■HELBg和HESBg分析检测到55种化合物。两种提取物都表现出毒性，包括在较高剂量HERBg下的胚胎凝固和在所有剂量HESBg下的胚胎中没有心跳。观察到行为变化；在最高剂量下发现成年斑马鱼的组织改变，主要在肝脏，肠,和肾脏，因为HELBg和HESBg的影响。HESBg的LD50为1717mg/kg，而HELBg超过2000mg/kg的限制剂量。
■广西双歧杆菌提取物的急性毒性研究显示出显著的毒性潜力，强调对胚胎和成年斑马鱼的影响。结果表明该物种制剂的相对安全性，鼓励关于潜在生物活性的进一步临床试验。
