近年来,人们对天然化合物的治疗潜力越来越感兴趣,特别是植物来源,由于它们的抗炎特性。其中,西药房室,通常被称为腰果,由于其公认的健康益处,已经引起了极大的关注。本研究旨在建立西麻提取物中所含的生物活性化合物与其抗炎活性之间的相关性。以干燥的西洋无心叶粉为提取基质。提取技术是浸渍,加压流体抽取(PFE),和超临界流体萃取(SFE)。通过LC-MS/MS进行提取物的初步分析。响应面法(RSM),主成分分析(PCA),和热图用于模拟实验条件对植物中特定化合物的提取率和峰面积的影响。为了评估抗炎活性,培养RAW264.7细胞,用LPS激活,并用不同浓度的植物提取物处理。使用XTT测定评估细胞增殖。的确,西西无心提取物含有无心酸,卡卡诺尔斯,还有Cardol,通过SFE和基于乙醇的方法产生不同的概况。RSM显示温度和乙醇,作为CO2的添加剂,显著影响PFE和SFE的提取效率。此外,这种具有SFE的组合物对特定组的化合物表现出更高的选择性。提取物在巨噬细胞中表现出抗炎特性而没有细胞毒性,降低活化细胞中促炎蛋白COX-2、COX-1和TLR4的水平。这表明它们作为抗炎剂的潜力,对未活化细胞中的细胞活力或促炎蛋白水平没有不利影响。总的来说,这些发现强调了西药无心提取物在减轻炎症方面的有希望的治疗潜力,同时也为优化目标化合物分离的提取过程提供了重要的见解。因此,这为该植物开发消炎药提供了良好的前景。
In recent years, there has been a growing interest in the
therapeutic potential of natural compounds, particularly of plant origin, owing to their demonstrated anti-inflammatory properties. Among these, Anacardium occidentale, commonly known as cashew, has garnered significant attention due to its reputed health benefits. This
study aim to establish a correlation between the bioactive compounds contained in the extracts of Anacardium occidentale and its anti-inflammatory activity. Dried Anacardium occidentale leaves powder was used as the extraction matrix. Extraction techniques are maceration, pressurized fluid extraction (PFE), and supercritical fluid extraction (SFE). The preliminary analysis of extracts was made by LC-MS/MS. The Response Surface Methodology (RSM), Principal Component Analysis (PCA), and heat maps were employed to model the influence of experimental conditions on extraction yield and peak area of specific compounds from the plant. To evaluate anti-inflammatory activity, RAW 264.7 cells were cultured, activated with LPS, and treated with varying concentrations of the plant extracts. Cell proliferation was assessed using the XTT assay. Indeed, Anacardium occidentale extracts contain anacardic acids, cardanols, and cardol, with distinct profiles yielded by SFE and ethanol-based methods. RSM shows that temperature and ethanol, as additives to CO2, significantly affect extraction efficiency in both PFE and SFE. Moreover, this composition with SFE demonstrate higher selectivity for specific group of compounds. The extracts exhibit anti-inflammatory properties without cytotoxicity in macrophages, reducing levels of pro-inflammatory proteins COX-2, COX-1, and TLR4 in activated cells. This suggests their potential as anti-inflammatory agents without adverse effects on cell viability or pro-inflammatory protein levels in non-activated cells. Overall, these findings underscore the promising
therapeutic potential of Anacardium occidentale extracts in mitigating inflammation, while also providing crucial insights into optimizing the extraction process for targeted compound isolation. Thus, this makes a good prospect for the development of anti-inflammatory drugs from this plant.