背景:塞浦路斯缺乏猫感染因子研究。这项研究的目的是确定各种猫科动物感染因子的患病率和危险因素,包括猫媒介传播病原体(FVBP),来自塞浦路斯的猫。
方法:横截面,描述性,描述性对174个猫科动物样本进行了多中心研究[拥有的138个样本和36个避难所,包括健康(43)和非健康(131),猫]来自塞浦路斯所有六个地区的私人兽医诊所。采用实时定量聚合酶链反应(qPCR)方法检测血支原体(Mhf),“念珠菌支原体痰”(CMhm)和“念珠菌支原体”(CMt)。对该人群进行了四种FVBP测试,包括鲍顿氏菌和利什曼原虫。使用qPCR,而常规PCR检测用于检测埃里希菌/无性体。和肝菌属。进行血清学测定以检测婴儿利什曼原虫抗体,猫白血病病毒(FeLV)抗原和猫免疫缺陷病毒(FIV)抗体。进行统计分析以测试变量与传染原之间的关联和可能的危险因素。
结果:174只猫中的96只(55.2%)对至少一种感染因子为PCR阳性。46只猫(26.4%)为血浆阳性,包括Mhf的13(7.5%),CMhm为36(20.7%),CMt为12(6.9%)。66只猫(37.9%)的肝菌属阳性。,而19例(10.9%)的HenselaeB.利什曼原虫属四(2.3%)。埃里希菌属/无性体属一个(0.6%)。测序显示存在肝虫动物,L.婴儿和无菌血症。在接受逆转录病毒血清学检查的164只猫中,10例(6.1%)为FeLV阳性,31例(18.9%)为FIV阳性,而在测试的160只猫中,有7只(4.4%)是阳性的。多变量逻辑回归显示,包括婴儿乳球菌在内的各种感染因子与每种肝虫菌均存在显着关联。和CMt感染。
结论:在来自塞浦路斯的Mhf猫中发现感染因子的高流行率,CMhm,CMt,L.婴儿,B.henselae,H.Felis,A.Platys,首次报道FeLV和FIV感染。不同病原体之间的显著关联提供了更好地理解这些病原体的流行病学相似性和它们之间的相互作用。
BACKGROUND: Feline infectious agent studies are lacking in Cyprus. The aims of this
study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (FVBP), in cats from Cyprus.
METHODS: A cross-sectional, descriptive, multicentre
study was performed on 174 feline samples [138 owned and 36 shelter-feral, including both healthy (43) and non-healthy (131), cats] from private veterinary clinics from all six districts of Cyprus. Real-time quantitative polymerase chain reaction (qPCR) assays were used to detect Mycoplasma haemofelis (Mhf), \"Candidatus Mycoplasma haemominutum\" (CMhm) and \"Candidatus Mycoplasma turicensis\" (CMt). The population was tested for four FVBP including Bartonella henselae and Leishmania spp. using qPCR, while conventional PCR assays were used to detect Ehrlichia/Anaplasma spp. and Hepatozoon spp. Serological assays were performed to detect Leishmania infantum antibodies, feline leukaemia virus (FeLV) antigen and feline immunodeficiency virus (FIV) antibodies. Statistical analysis was performed to test associations and possible risk factors between variables and infectious agents.
RESULTS: Ninety-six (55.2%) of the 174 cats were PCR-positive for at least one infectious agent. Forty-six cats (26.4%) were haemoplasma positive, including 13 (7.5%) for Mhf, 36 (20.7%) for CMhm and 12 (6.9%) for CMt. Sixty-six cats (37.9%) were positive for Hepatozoon spp., while 19 (10.9%) were positive for B. henselae, four (2.3%) for Leishmania spp. and one (0.6%) for Ehrlichia/Anaplasma spp. Sequencing revealed the presence of Hepatozoon felis, L. infantum and Anaplasma platys. Of the 164 cats that underwent retroviral serology, 10 (6.1%) were FeLV-positive and 31 (18.9%) were FIV-positive, while L. infantum serology was positive in 7 (4.4%) of the 160 cats tested. Multivariable logistic regression revealed significant associations for various infectious agents including L. infantum with each of Hepatozoon spp. and CMt infection.
CONCLUSIONS: A high prevalence of infectious agents was found in cats from Cyprus with Mhf, CMhm, CMt, L. infantum, B. henselae, H. felis, A. platys, FeLV and FIV infections reported for the first time. The significant associations between different pathogens provide a better understanding of similarities in the epidemiology of these pathogens and interactions between them.