UNASSIGNED: The widespread use of radiofrequency (RF) sources, ranging from household appliances to telecommunications devices and military equipment, raises concerns among people and regulatory agencies about the potential health risks of RF exposure. Consequently, several in vitro and in vivo studies have been done to investigate the biological effects, in particular non-thermal, of this non-ionizing radiation. To date, this issue is still being debated due to the controversial results that have been reported. Furthermore, the impact of different RF signal modulations on biological systems remains poorly investigated. The present in vitro study aims to evaluate the cytotoxicity and genotoxicity of continuous or pulsed 1.6 GHz RF in human dermal fibroblasts (HDF).
UNASSIGNED: HDF cultures were exposed to continuous and pulsed 1.6 GHz RF, for 2 h, with Specific Absorption Rate (SAR) of 0.4 W/kg. The potential biological effects of 1.6 GHz RF on HDF were assessed with a multi-methodological approach, analyzing the effects on cell cycle, ultrastructure, protein expression, mitotic spindle, CREST stained micronuclei, chromosome segregation and γ-H2AX/53BP1 foci.
UNASSIGNED: 1.6 GHz RF exposure modified proteins expression and morphology of HDF. Specifically, the expression of different heat-shock proteins (HSP) (i.e., HSP-90, HSP-60, and HSP-25) and phospho-AKT were affected. In addition, both continuous and pulsed RF modified the cytoskeletal organization in HDF and increased the number of lysosomes, while the formation of autophagosomes was observed only after pulsed RF exposure. Mitotic spindle anomalies were also found after exposure. However, no significant effect was observed on cell cycle, chromosome segregation, CREST-stained micronuclei and γ-H2AX/53BP1 foci.
UNASSIGNED: The results of the present study show the absence of genotoxic damage in 1.6 GHz RF exposed HDF and, although mitotic spindle alterations were observed, they did not have an aneugenic effect. On the other hand, changes in some proteins expression and cell ultrastructure in exposed HDF suggest that RF can potentially induce cell alterations at the morphological and molecular levels.

Thermal processing is commonly employed to ensure the quality and extend the shelf-life of fruits and vegetables. Radio frequency (RF) heating has been used as a promising alternative treatment to replace conventional thermal processing methods with advantages of rapid, volumetric, and deep penetration heating characteristics. This article provides comprehensive information regarding RF heating uniformity and applications in processing of fruit and vegetable products, including disinfestation, blanching, drying, and pasteurization. The dielectric properties of fruits and vegetables and their products have also been summarized. In addition, recommendations for future research on RF heating are proposed to enhance practical applications for fruits and vegetables processing in future.

The objective were to evaluate the effects of supplementation of standardized dry extract of Rosmarinus officinalis (RO) and the application of aesthetic radiofrequency on the oxidative stress markers catalase (CAT), superoxide dismutase (SOD), non-protein thiols (NP-SH), and thiobarbituric acid reactive species (TBARS) and the biochemical markers triglycerides, total cholesterol, high density lipoprotein (HDL) cholesterol, glutamic-oxaloacetic transaminase (TGO/AST), pyruvic-glutamic transaminase (TGP/ALT), gamma glutamyl transpeptidase (gamma-GT), and creatinine. This study included 32 women received the aesthetic therapy to reduce localized fat. They were divided into the control group (n = 8) receiving placebo capsules and the intervention group (n = 24) subdivided into Group A, B, and C, each with eight members receiving supplementation with 100, 500, and 1000 mg/day of standardized dry extract of RO, respectively. The Universal Trial Number (UTN) - U1111-1274-6255. Supplementation with RO (500 mg/day) demonstrated a reduction in oxidative stress (quantified with through a significant increase in NP-SH and a reduction in SOD and CAT enzymes). The radiofrequency aesthetic treatment did not promote an increase in oxidative stress; however, it caused significant changes in total cholesterol, HDL cholesterol, and creatinine. RO is a plant with antioxidant effects and its oral consumption is safe in selected women subjects in hepatic and renal markers.

Cognitive radio networks (CRN) enable wireless devices to sense the radio spectrum, determine the frequency state channels, and reconfigure the communication variables to satisfy Quality of Service (QoS) needs by reducing energy utilization. In CRN, spectrum sensing is an essential process that is highly challenging and can be addressed by several traditional techniques, such as energy detection, match filtering, etc. For now, the current models\' performance is impacted by the comparatively low Signal to Noise Ratio (SNR) of recognized signals and the insignificant quantity of traditional signal samples. This research proposals a new spectral sensing technique for cognitive radio networks (SST-CRN) that addresses the drawbacks of predictable energy detection models. With the use of a deep belief network (DBN), the suggested model contributes to accomplish a nonlinear threshold based on the chicken swarm algorithm (CSA). The proposed DBN enabled SST-CRN technique goes through two phases in a organized process: offline and online. Throughout the offline phase, the DBN model is methodically trained on pre-gathered data, developing the aptitude to identify problematic patterns and examples from the spectral features of the radio environment. This stage involves extensive feature extraction, validation, and model development to ensure that the DBN can professionally represent complicated spectral dynamics. Additionally, online spectrum sensing is conducted during the real communication phase to enable real-time adaptation to dynamic changes in the spectrum environment. Offline spectrum sensing is typically performed during a devoted sensing period before actual communication begins. When combined with DBN\'s deep learning capabilities and CSO\'s innate nature-inspired algorithms, a synergistic framework is created that enables CRNs to explore and allocate incidences on their own with astonishing accuracy. The proposed solution considerably improves the spectrum efficiency and resilience of CRNs by harnessing the power of DBN, which leads to more effective resource utilization and less interference. The Simulation results show that our proposed strategy produces more accurate spectrum occupancy assessments. The result parameters such as probability of detection, SNR of -24dB, the SST-CRN perfect has increased a developed Pd of 0.810, whereas the existing methods RMLSSCRN-100 and RMLSSCRN-300 have accomplished a lower Pd of 0.577 and 0.736, respectively. Our deep learning methodology uses convolutional neural networks to automatically learn and adapt to dynamic and complicated radio environments, improving accuracy and flexibility over classic spectrum sensing approaches. Future research might focus on improving CSO algorithms to better optimize the spectrum sensing process, enhancing the reliability of DBN-enabled sensing techniques.

The magnetic compass sense of migratory songbirds is thought to derive from magnetically sensitive photochemical reactions in cryptochromes located in photoreceptor cells in the birds\' retinas. More specifically, transient radical pairs formed by light-activation of these proteins have been proposed to account for the birds\' ability to orient themselves using the Earth\'s magnetic field and for the observation that radiofrequency magnetic fields, superimposed on the Earth\'s magnetic field, can disrupt this ability. Here, by means of spin dynamics simulations, we show that it may be possible for the birds to orient in a monochromatic radiofrequency field in the absence of the Earth\'s magnetic field. If such a behavioural test were successful, it would provide powerful additional evidence for a radical pair mechanism of avian magnetoreception.

The deployment of 5 G wireless technology has generated considerable interest and debate regarding its potential effects on human health. This work provides a comprehensive overview of the current scientific understanding of the potential health implications associated with 5 G technology. Drawing upon a wide range of research studies, reviews, and expert opinions, we explore the implications through which 5 G signals interact with the human body. This work presents a balanced perspective, summarizing both the potential benefits of 5 G technology, such as improved data transfer speeds, reduced latency, and enhanced connectivity, as well as concerns that have been raised about its effects on human\'s tissues. We discuss various aspects of health impacts, including thermal and nonthermal effects, focusing on the existing research on radiofrequency electromagnetic fields and their potential to cause adverse health outcomes. Simulation results show the negative effect of radio waves on human\'s tissues.

Objective.A bone-inclusive ASTM phantom is proposed to improve the assessment of radiofrequency electromagnetic field (RF-EMF) power deposition near orthopedic device under 1.5 T and 3 T magnetic resonance imaging (MRI).Approach.A phantom is created by introducing a cylindrical bone structure inside the American Society for Testing and Materials (ASTM) phantom. Four orthopaedic implant families-rod, nailing system, plate system, and hip replacement-are used in the study. RF-EMF power deposition (in terms of peak averaged specific absorption rate over 1 gram) near these implants are evaluated by placing these implants inside the standard ASTM phantom, the developed bone-inclusive ASTM phantom, and two anatomically representative human body phantoms, known as Duke and Ella. Numerical simulations are performed to calculate the RF-EMF power deposition near various orthopaedic devices within these phantoms.Main Results.For devices implanted inside or near bone tissue, the evaluation of RF-EMF power deposition using the developed bone-inclusive ASTM phantom shows better correlations to the human body phantoms than the ASTM phantom. This improvement is attributed to the portion of the devices implanted within the bone tissue.Significance.The bone-inclusive ASTM phantom has the different tissue of interests surrounding the implants compared to the ASTM phantom. This variation can lead to the different resonance frequency under RF-EMF exposure. This leads to better correlation of RF-EMF power deposition near orthopaedic implants inside human body, making the bone-inclusive ASTM phantom more suitable for evaluating RF-EMF power deposition than ASTM phantom in MRI scans.

Androgenic alopecia (AGA) is the most common type of alopecia and its treatments involve drugs that have various adverse effects and are not completely effective. Radiofrequency-based therapies (RF) are an alternative for AGA treatment. Although there is increasing clinical evidence of the effectiveness of RF for alopecia, its effects at the tissue and cellular level have not been studied in detail. The objective of this study was to analyze ex vivo the potential effect of RF currents used in capacitive resistive electrical transfer (CRET) therapy on AGA. Hair follicles (HFs) were donated by patients with AGA and treated with CRET. AGA-HFs were exposed in vitro to intermittent 448 kHz electric current in subthermal conditions. Cell proliferation (Ki67), apoptosis (TUNEL assay), differentiation (β-catenin), integrity (collagen and MMP9), thickness of the epidermis surrounding HF, proportion of bulge cells and melanoblasts in AGA-HF were analyzed by immunohistochemistry. CRET increased proliferation and decreased death of different populations of AGA-HF cells. In addition, the melanoblasts increased in bulge and the epidermis surrounding the hair follicle thickened. These results support the effectiveness of RF-based therapies for the treatment of alopecia. However, clinical trials are necessary to know the true effectiveness of CRET therapy and other RF therapies for AGA treatment.

Recent years, the rapid advancement of technology has raised concerns. We studied the effects of prenatal exposure to 900 MHz radiofrequency (RF) from mobile phones and the protective effects of linalool on learning and memory, and anxiety in adolescent male and female offspring rats. Pregnant rats were divided into four groups: control, wave, wave + linalool, and linalool. Rats received linalool (25mg/kg) by gavage for 21 days. Irradiation was conducted from day 0 to day 21 of pregnancy. Offsprings underwent behavioral and electrophysiological tests on days 50 and 60 after birth. Exposure to RF during pregnancy caused anxiety-like behavior in the EPM test and impairment of learning and memory in the Morris water maze and shuttle box tests. Electrophysiological properties and synaptic plasticity of the dorsal hippocampal CA3-CA1 synapse showed a decrease in fEPSP amplitude and slope. The trace element levels in both male and female offspring were consistent across all groups compared to their respective controls. In the hippocampus tissue, the levels of Fe, Cu, and Mn, as well as the Cu/Zn ratio, were significantly higher in the exposed groups (wave groups) compared to their controls. Moreover, Zn levels were significantly lower in the hippocampus tissue of the exposed groups. Linalool administration mitigated the excessive increase in Fe, Cu, Mn, and Cu/Zn ratio and normalized the disrupted levels of trace elements, except for Zn levels in both male and female offspring. Sex differences were observed in the EPM and shuttle box tests, females were more sensitive than males. In summary, our study demonstrates that prenatal exposure to mobile phone radiation induces stress-like behaviors, disrupts learning and memory, alters hippocampal electrophysiological properties and trace element balance in offspring. Treatment with linalool mitigates these deleterious effects, highlighting its potential as a therapeutic intervention. These findings contribute to our understanding of the impact of prenatal environmental exposures on neurodevelopment and offer insights into potential strategies for neuroprotection.

This paper presents the design and isolation enhancement of a filtering MIMO antenna with a radiation null for out-of-band suppressions suited for 5G sub-6 GHz communications. The MIMO antenna offers -10 dB impedance bandwidth functionality at the most prominent partial spectrum of the 5G NR n78 band for enabling wireless applications in base stations, ranging from 3.4 GHz to 3.61 GHz. To mitigate the redundancy of an RF filter and to achieve a strong filtering response, a radiation null is produced in the gain with four identical rectangular slots, which results in a significant gain drop of more than 8 dBi at the stopband. The geometrical design also allows 30 percent size reduction of single element. Subsequently, a closely spaced (0.11λ0) two-port MIMO antenna is implemented and with the utilization of the proposed rectangular shaped hollow stub parasitic element, the interelement isolation is significantly improved by more than 8 dB over the operational frequency range while retaining the filtering without any additional RF structure. The design simplification, peak gain of 5.4 dBi, near ideal response of diversity gain, ECC less than 0.03, congruency between simulated and measured results, and stable parameters make it a valuable choice for 3.5 GHz sub-6 GHz communications.