PDF(Pubmed)
Abstract：
Androgenic alopecia (AGA) is the most common type of alopecia and its treatments involve drugs that have various adverse effects and are not completely effective. Radiofrequency-based therapies (RF) are an alternative for AGA treatment. Although there is increasing clinical evidence of the effectiveness of RF for alopecia, its effects at the tissue and cellular level have not been studied in detail. The objective of this study was to analyze ex vivo the potential effect of RF currents used in capacitive resistive electrical transfer (CRET) therapy on AGA. Hair follicles (HFs) were donated by patients with AGA and treated with CRET. AGA-HFs were exposed in vitro to intermittent 448 kHz electric current in subthermal conditions. Cell proliferation (Ki67), apoptosis (TUNEL assay), differentiation (β-catenin), integrity (collagen and MMP9), thickness of the epidermis surrounding HF, proportion of bulge cells and melanoblasts in AGA-HF were analyzed by immunohistochemistry. CRET increased proliferation and decreased death of different populations of AGA-HF cells. In addition, the melanoblasts increased in bulge and the epidermis surrounding the hair follicle thickened. These results support the effectiveness of RF-based therapies for the treatment of alopecia. However, clinical trials are necessary to know the true effectiveness of CRET therapy and other RF therapies for AGA treatment.
摘要：
雄激素性脱发（AGA）是最常见的脱发类型，其治疗涉及具有各种不良反应且不完全有效的药物。基于射频的疗法(RF)是AGA治疗的替代方案。尽管有越来越多的临床证据表明射频治疗脱发的有效性，其在组织和细胞水平的影响尚未详细研究。这项研究的目的是分析体外电容电阻电传输（CRET）治疗中使用的RF电流对AGA的潜在影响。毛囊（HF）由AGA患者捐赠，并接受CRET治疗。在亚热条件下将AGA-HFs在体外暴露于间歇性448kHz电流。细胞增殖（Ki67），凋亡（TUNEL测定），分化（β-连环蛋白），完整性（胶原蛋白和MMP9），HF周围表皮的厚度，通过免疫组织化学分析AGA-HF中隆起细胞和黑素细胞的比例。CRET增加了不同群体的AGA-HF细胞的增殖并减少了死亡。此外,黑素细胞隆起增加，毛囊周围的表皮增厚。这些结果支持基于RF的疗法用于治疗脱发的有效性。然而,临床试验对于了解CRET疗法和其他RF疗法对AGA治疗的真正有效性是必要的.
