热应激(HS)影响核蛋白质组,随后,不同核室中的蛋白质活性。在拟南芥中,短时间暴露于37°C会导致核仁的标准三方结构丧失,最突出的核子结构,and,因此,影响核糖体的组装。这里,我们报告了定量的无标记LC-MS/MS(液相色谱与串联质谱联用)分析,以确定拟南芥在22°C时的核蛋白质组,HS(37°C,4和24小时),和恢复阶段。该分析根据之前细胞核中的相对丰度变化确定了十组不同的蛋白质,HS期间和之后:早期,迟到,瞬变,早期的持久性,后期持续,Recovery,早期喜欢,Late-like,类瞬态和连续组(EG,LG,TG,EPG,液化石油气,RG,ELG,LLG,TLG和CG,分别)。有趣的是,RNA聚合酶I亚基NRPA3和其他主要核仁蛋白,在RG和CG中检测到包括核蛋白1和纤丝蛋白1和2,表明植物需要增加的核仁活性和可能的核糖体组装来恢复HS后的蛋白质合成。
Heat stress (HS) impacts the nuclear proteome and, subsequently, protein activities in different nuclear compartments. In Arabidopsis thaliana, a short exposure to 37 °C leads to loss of the standard tripartite architecture of the
nucleolus, the most prominent nuclear substructure, and, consequently, affects the assembly of ribosomes. Here, we report a quantitative label-free LC‒MS/MS (Liquid Chromatography coupled to tandem Mass Spectrometry) analysis to determine the nuclear proteome of Arabidopsis at 22 °C, HS (37 °C for 4 and 24 h), and a recovery phase. This analysis identified ten distinct groups of proteins based on relative abundance changes in the nucleus before, during and after HS: Early, Late, Transient, Early Persistent, Late Persistent, Recovery, Early-Like, Late-Like, Transient-Like and Continuous Groups (EG, LG, TG, EPG, LPG, RG, ELG, LLG, TLG and CG, respectively). Interestingly, the RNA polymerase I subunit NRPA3 and other main nucleolar proteins, including NUCLEOLIN 1 and FIBRILLARIN 1 and 2, were detected in RG and CG, suggesting that plants require increased nucleolar activity and likely ribosome assembly to restore protein synthesis after HS.