■在不同的热带和亚热带国家,对热带布洛米的敏感与哮喘有关;然而,与这种疾病相关的特定分子成分的信息很少。利用分子诊断,我们试图在哥伦比亚鉴定与哮喘相关的热带B变应原.
方法:使用内部ELISA系统在几个哥伦比亚城市(Barranquilla,Barranquilla,波哥大,麦德林,卡利,和圣安德烈斯)。研究样本包括儿童和成人(平均[SD]年龄,28[17]年)。使用ELISA抑制评估Blot5和Blot21之间的交叉反应性。
结果:使用内部ELISA系统在几个哥伦比亚城市进行的一项全国患病率研究中招募的哮喘患者(n=272)和对照组(n=298)确定了8个热带B重组变应原(Blot2、5、7、8、10、12、13和21)的特异性IgE(sIgE)。波哥大,麦德林,卡利,和圣安德烈斯)。研究样本包括儿童和成人(平均[SD]年龄,28[17]年)。使用ELISA抑制评估Blot5和Blot21之间的交叉反应性。
结论:尽管Blot5和Blot21被认为是常见的致敏剂,这是他们与哮喘的相关性的第一份报告.这两种成分都应包括在分子组中,以诊断热带地区的过敏。
UNASSIGNED: Sensitization to Blomia tropicalis is associated with asthma in various tropical and subtropical countries; however, information about the specific molecular components associated with this disease is scarce. Using molecular diagnosis, we sought to identify B tropicalis allergens associated with asthma in Colombia.
METHODS: Specific IgE (sIgE) to 8 B tropicalis recombinant allergens (Blo t 2, 5, 7, 8, 10, 12, 13, and 21) was determined using an in-house ELISA system in asthma patients (n=272) and controls (n=298) recruited in a national prevalence
study performed in several Colombian cities (Barranquilla, Bogotá, Medellín, Cali, and San Andrés). The
study sample included children and adults (mean [SD] age, 28 [17] years). Cross-reactivity between Blo t 5 and Blo t 21 was evaluated using ELISA-inhibition.
RESULTS: Specific IgE (sIgE) to 8 B tropicalis recombinant allergens (Blo t 2, 5, 7, 8, 10, 12, 13, and 21) was determined using an in-house ELISA system in asthma patients (n=272) and controls (n=298) recruited in a national prevalence
study performed in several Colombian cities (Barranquilla, Bogotá, Medellín, Cali, and San Andrés). The
study sample included children and adults (mean [SD] age, 28 [17] years). Cross-reactivity between Blo t 5 and Blo t 21 was evaluated using ELISA-inhibition.
CONCLUSIONS: Although Blo t 5 and Blo t 21 are considered common sensitizers, this is the first report of their association with asthma. Both components should be included in molecular panels for diagnosis of allergy in the tropics.