本系统综述的目的是评估有关正畸治疗(OT)患者口腔粘膜细胞中微核试验的已发表论文。在PubMed中搜索了科学文献,Scopus,和WebofScience数据库的所有数据直到11月发布,2021年使用以下关键词的组合:“固定正畸治疗,\"\"遗传损伤\",“DNA损伤,\"\"遗传毒性\",“致突变性”,“口腔细胞”,“口腔粘膜细胞,\"和\"微核试验\"。系统评价是根据系统评价和荟萃分析(PRISMA)指南的首选报告项目设计的。检索了9项研究。一些作者证明OT诱导口腔粘膜细胞的细胞遗传学损伤。在九项研究中,两个被归类为强壮,五个适度,和两个一样弱,根据有效公共卫生实践项目(EPHPP)的质量评估组成部分。荟萃分析数据显示,在不同的治疗月中,口腔细胞的诱变性与OT之间没有关系。一个月后,SMD=0.65和p=0.08;经过三个月的OT,SMD=1.21和p=0.07;经过六个月的OT,SMD=0.56和p=0.11。在OT的分析月份中,I2值>75%,表明高度异质性。总之,本综述未能证明OT诱导口腔细胞的遗传损伤.该研究对于进行固定OT的患者的保护具有重要意义,鉴于诱变参与了多步骤的致癌过程。
The aim of this systematic
review was to evaluate published papers regarding the micronucleus assay in oral mucosal cells of patients undergoing orthodontic therapy (OT). A search of the scientific literature was made in the PubMed, Scopus, and Web of Science databases for all data published until November, 2021 using the combination of the following keywords: \"fixed orthodontic therapy,\" \"genetic damage\", \"DNA damage,\" \"genotoxicity\", \"mutagenicity\", \"buccal cells\", \"oral mucosa cells,\" and \"micronucleus assay\". The systematic
review was designed according to the Preferred Reporting Items for Systematic
Review and Meta-Analyses (PRISMA) guidelines. Nine studies were retrieved. Some authors demonstrated that OT induces cytogenetic damage in oral mucosal cells. Out of the nine studies included, two were classified as strong, five as moderate, and two as weak, according to the quality assessment components of the Effective Public Health Practice Project (EPHPP). Meta-analysis data revealed no relationship between mutagenicity in oral cells and OT in different months of treatment. At one month, the SMD = 0.65 and p = 0.08; after three months of OT, the SMD = 1.21 and p = 0.07; and after six months of OT, the SMD = 0.56 and p = 0.11. In the analyzed months of OT, I2 values were >75%, indicating high heterogeneity. In summary, this
review was not able to demonstrate that OT induces genetic damage in oral cells. The study is important for the protection of patients undergoing fixed OT, given that mutagenesis participates in the multi-step process of carcinogenesis.