背景:干细胞疗法被认为在可用于再生牙科的治疗方式中起着至关重要的作用。在细胞水平上建立的光生物调节(PBM)的有益效果已经导致这两种因子(PBM和干细胞)的组合使用。本研究的主要目的是首先批判性地评价PBM对牙周膜干细胞(PDLSCs)的影响,其次探索应用最有效的PBM协议。
方法:发布,科克伦,Scopus,科学直接,和GoogleScholar搜索引擎用于确定将PBM应用于培养的PDLSCs的实验性体外研究。应用特定关键字后,额外的过滤器,和纳入/排除标准,245件的初步数量缩小到11件,其中激光器和LED在630-1064nm波长范围内使用。三位独立审稿人进一步评估了选定的文章是否严格遵守PRISMA准则,以及修改后的Cochrane偏差风险来确定资格。
方法:分析的数据集从研究中提取,并具有充分且清晰的PBM方案。进行了简单的单变量回归分析,以探讨潜在的定量预测变量对研究结果的贡献的意义。并且采用单向方差分析模型来测试治疗的激光或LED源之间的差异。测试的显著性水平设定为α=0.05。
结果:增殖率,成骨分化,发现不同的成骨和炎症抑制指示基因的表达受到各种类型的激光和LED的应用的积极影响。关于PBM协议,只有波长变量似乎会影响治疗结果;事实上,940nm波长参数被发现没有发挥有利的作用。
结论:光生物调节可增强牙周膜干细胞的干性和分化能力。因此,对于PBM协议,科学界没有达成共识。这里进行的统计分析表明,采用940nm的近红外(NIR)波长可能不会产生明显的有利结果。尽管那些在630-830nm范围内的人这样做了。关于通量,当通过LED设备进行治疗时,它不应该超过8J/cm2,和4J/cm2当分别由激光施加。
BACKGROUND: Stem cell therapy has been considered to play a paramount role in the treatment modalities available for regenerative dentistry. The established beneficial effects of photobiomodulation (PBM) at the cellular level have
led to the combined use of these two factors (PBM and stem cells). The main goal of this study was firstly to critically appraise the effects of PBM on periodontal ligament stem cells (PDLSCs), and secondly to explore the most effective PBM protocols applied.
METHODS: Pubmed, Cochrane, Scopus, Science Direct, and Google Scholar search engines were used to identify experimental in vitro studies in which PBM was applied to cultured PDLSCs. After applying specific keywords, additional filters, and inclusion/exclusion criteria, a preliminary number of 245 articles were narrowed down to 11 in which lasers and LEDs were used within the 630 - 1064 nm wavelength range. Selected articles were further assessed by three independent reviewers for strict compliance with PRISMA guidelines, and a modified Cochrane risk of bias to determine eligibility.
METHODS: The dataset analysed was extracted from the studies with sufficient and clearly presented PBM protocols. Simple univariate regression analysis was performed to explore the significance of contributions of potential quantitative predictor variables toward study outcomes, and a one-way ANOVA model was employed for testing differences between the laser or
LED sources of the treatments. The significance level for testing was set at α = 0.05.
RESULTS: The proliferation rate, osteogenic differentiation, and expression of different indicative genes for osteogenesis and inflammation suppression were found to be positively affected by the application of various types of lasers and LEDs. With regard to the PBM protocol, only the wavelength variable appeared to affect the treatment outcome; indeed, the 940 nm wavelength parameter was found not to exert a favourable effect.
CONCLUSIONS: Photobiomodulation can enhance the stemness and differentiation capacities of periodontal ligament stem cells. Therefore, for PBM protocols, there remains no consensus amongst the scientific community. Statistical analyses performed here indicated that the employment of a near-infrared (NIR) wavelength of 940 nm may not yield a significant favourable outcome, although those within the 630 - 830 nm range did so. Concerning the fluence, it should not exceed 8 J/cm2 when therapy is applied by
LED devices, and 4 J/cm2 when applied by lasers, respectively.