本研究旨在研究赤霉酸(GBA)对生长的影响,与肝功能相关的血液生化参数,消化酶,和尼罗罗非鱼的免疫反应。此外,通过评估抗氧化剂,探索了可能的潜在机制,凋亡,和免疫相关基因表达。此外,研究了补充α-硫辛酸(LIP)膳食后恢复的可能性.鱼(平均初始体重30.75±0.46)被平均分为四组:对照组,LIP组(以基础饮食加600mg/kgLIP为食),GBA组(暴露于150mgGBA/L),和GBA+LIP组(暴露于150mgGBA/L并饲喂含有LIP和GBA的饮食)60天。研究结果表明,补充LIP显著降低了GBA对存活率的有害影响,增长,饲料摄入量,消化酶,和抗氧化平衡。此外,GBA暴露显著增加肝酶,应力标记,胆固醇,和甘油三酯水平,所有这些都通过补充LIP有效缓解。此外,在鱼类饮食中添加LIP可显着减少GBA处理的鱼类肝脏的组织病理学改变,包括脂肪变化,清晰的细胞质,核转移到细胞外围,单细胞坏死,血管充血,和小叶内出血.GBA诱导的溶菌酶活性降低,补体C3和一氧化氮水平,连同抗氧化剂基因(猫和sod)的下调,通过饮食LIP显着恢复。同时,将LIP添加到GBA暴露的鱼食中,显着纠正了hsp70,caspase-3,P53,pcna的异常表达,tnf-a,和il-1β在尼罗氏肝脏中。最后,膳食LIP补充可以减轻GBA暴露对鱼类生长和性能的有害影响,生理条件,先天免疫,抗氧化能力,炎症反应,和细胞凋亡。
This
study aimed to examine the effects of gibberellic acid (GBA) on growth, hemato-biochemical parameters related to liver functions, digestive enzymes, and immunological response in Oreochromis niloticus. Besides, the probable underlying mechanisms were explored by assessing antioxidant, apoptotic, and immune-related gene expression. Furthermore, the likelihood of restoration following alpha-lipoic acid (LIP) dietary supplementation was explored. The fish (average initial weight 30.75 ± 0.46) were equally classified into four groups: the control group, the LIP group (fed on a basal diet plus 600 mg/kg of LIP), the GBA group (exposed to 150 mg GBA/L), and the GBA + LIP group (exposed to 150 mg GBA/L and fed a diet containing LIP and GBA) for 60 days. The
study findings showed that LIP supplementation significantly reduced GBA\'s harmful effects on survival rate, growth, feed intake, digestive enzymes, and antioxidant balance. Moreover, the GBA exposure significantly increased liver enzymes, stress markers, cholesterol, and triglyceride levels, all of which were effectively mitigated by the supplementation of LIP. Additionally, LIP addition to fish diets significantly minimized the histopathological alterations in the livers of GBA-treated fish, including fatty change, sharply clear cytoplasm with nuclear displacement to the cell periphery, single-cell necrosis, vascular congestion, and intralobular hemorrhages. The GBA-induced reduction in lysozyme activity, complement C3, and nitric oxide levels, together with the downregulation of antioxidant genes (cat and sod), was significantly restored by dietary LIP. Meanwhile, adding LIP to the GBA-exposed fish diets significantly corrected the aberrant expression of hsp70, caspase- 3, P53, pcna, tnf-a, and il-1β in O. niloticus liver. Conclusively, dietary LIP supplementation could mitigate the harmful effects of GBA exposure on fish growth and performance, physiological conditions, innate immunity, antioxidant capability, inflammatory response, and cell apoptosis.