Abstract：
BACKGROUND: Acute lung injury (ALI) is a clinical syndrome characterized by pulmonary inflammation. Ultrashort wave diathermy (USWD) has been shown to be effective at in inhibiting ALI inflammation, although the underlying mechanism remains unclear. Previous studies have demonstrated that USWD generates a therapeutic thermal environment that aligns with the temperature required for heat shock protein 70 (HSP70), an endogenous protective substance. In this study, we examined the correlation between HSP70 and USWD in alleviating lung inflammation in ALI.
METHODS: Forty-eight male C57BL/6 mice were randomly divided into control, model, USWD intervention (LU) 1, 2, and 3, and USWD preintervention (UL) 1, 2, and 3 groups (n = 6 in each group). The mice were pretreated with LPS to induce ALI. The UL1, 2, and 3 groups received USWD treatment before LPS infusion, while the LU1, 2, and 3 groups received USWD treatment after LPS infusion. Lung function and structure, inflammatory factor levels and HSP70 protein expression levels were detected.
RESULTS: USWD effectively improved lung structure and function, and significantly reduced IL-1β, IL-10, TGF-β1, and TNF-α levels in both the USWD preintervention and intervention groups. However, HSP70 expression did not significantly differ across the experimental groups although the expression of TLR4 was significantly decreased, suggesting that USWD may have anti-inflammatory effects through multiple signaling pathways or that the experimental conditions should be restricted.
CONCLUSIONS: Both USWD intervention and preintervention effectively reduced the inflammatory response, alleviated lung injury symptoms, and played a protective role in LPS-pretreated ALI mice. HSP70 was potentially regulated by USWD in this process, but further studies are urgently needed to elucidate the correlation and mechanism.
METHODS: Forty-eight male C57BL/6 mice were randomly divided into control, model, USWD intervention (LU) 1, 2, and 3, and USWD preintervention (UL) 1, 2, and 3 groups (n = 6 in each group). The mice were pretreated with LPS to induce ALI. The UL1, 2, and 3 groups received USWD treatment before LPS infusion, while the LU1, 2, and 3 groups received USWD treatment after LPS infusion. Lung function and structure, inflammatory factor levels and HSP70 protein expression levels were detected.
RESULTS: USWD effectively improved lung structure and function, and significantly reduced IL-1β, IL-10, TGF-β1, and TNF-α levels in both the USWD preintervention and intervention groups. However, HSP70 expression did not significantly differ across the experimental groups although the expression of TLR4 was significantly decreased, suggesting that USWD may have anti-inflammatory effects through multiple signaling pathways or that the experimental conditions should be restricted.
CONCLUSIONS: Both USWD intervention and preintervention effectively reduced the inflammatory response, alleviated lung injury symptoms, and played a protective role in LPS-pretreated ALI mice. HSP70 was potentially regulated by USWD in this process, but further studies are urgently needed to elucidate the correlation and mechanism.
摘要：
背景：急性肺损伤（ALI）是一种以肺部炎症为特征的临床综合征。超短波透热疗法（USWD）已被证明可有效抑制ALI炎症，尽管潜在的机制尚不清楚.先前的研究表明，USWD产生的治疗热环境与热休克蛋白70（HSP70）所需的温度一致，内源性保护性物质。在这项研究中,我们研究了HSP70和USWD在减轻ALI肺部炎症中的相关性。
方法：48只雄性C57BL/6小鼠随机分为对照组，模型，USWD干预（LU）1、2和3，以及USWD干预前（UL）1、2和3组（每组n=6）。用LPS预处理小鼠以诱导ALI。UL1、2和3组在LPS输注前接受USWD治疗，而LU1、2和3组在LPS输注后接受USWD治疗。肺功能和结构，检测炎症因子水平和HSP70蛋白表达水平。
结果：USWD有效改善了肺结构和功能，并显著降低IL-1β，USWD干预前和干预组的IL-10，TGF-β1和TNF-α水平。然而,尽管TLR4的表达显著降低,但HSP70在各实验组中的表达没有显著差异,提示USWD可能通过多种信号传导途径具有抗炎作用，或者应限制实验条件。
结论：USWD干预和干预前都能有效降低炎症反应，减轻肺损伤症状，并在LPS预处理的ALI小鼠中发挥保护作用。HSP70在此过程中可能受到USWD的调控，但迫切需要进一步的研究来阐明相关性和机制。
方法：48只雄性C57BL/6小鼠随机分为对照组，模型，USWD干预（LU）1、2和3，以及USWD干预前（UL）1、2和3组（每组n=6）。用LPS预处理小鼠以诱导ALI。UL1、2和3组在LPS输注前接受USWD治疗，而LU1、2和3组在LPS输注后接受USWD治疗。肺功能和结构，检测炎症因子水平和HSP70蛋白表达水平。
结果：USWD有效改善了肺结构和功能，并显著降低IL-1β，USWD干预前和干预组的IL-10，TGF-β1和TNF-α水平。然而,尽管TLR4的表达显著降低,但HSP70在各实验组中的表达没有显著差异,提示USWD可能通过多种信号传导途径具有抗炎作用，或者应限制实验条件。
结论：USWD干预和干预前都能有效降低炎症反应，减轻肺损伤症状，并在LPS预处理的ALI小鼠中发挥保护作用。HSP70在此过程中可能受到USWD的调控，但迫切需要进一步的研究来阐明相关性和机制。
