PDF(Pubmed)
Abstract：
BACKGROUND: Heat shock protein 70 (HSP70) exhibits protective effects against ultraviolet (UV)-induced premature skin aging. A standardized extract of Asparagus officinalis stem (EAS) is produced as a novel and unique functional food that induces HSP70 cellular expression. To elucidate the anti-photoaging potencies of EAS, we examined its effects on HSP70 expression levels in UV-B-irradiated normal human dermal fibroblasts (NHDFs).
METHODS: NHDFs were treated with 1 mg/mL of EAS or dextrin (vehicle control) prior to UV-B irradiation (20 mJ/cm2). After culturing NHDFs for different time periods, HSP70 mRNA and protein levels were analyzed using real-time polymerase chain reaction and western blotting, respectively.
RESULTS: UV-B-irradiated NHDFs showed reduced HSP70 mRNA levels after 1-6 h of culture, which were recovered after 24 h of culture. Treatment with EAS alone for 24 h increased HSP70 mRNA levels in the NHDFs, but the increase was not reflected in its protein levels. On the other hand, pretreatment with EAS abolished the UV-B irradiation-induced reduction in HSP70 expression at both mRNA and protein levels. These results suggest that EAS is capable to preserve HSP70 quantity in UV-B-irradiated NHDFs.
CONCLUSIONS: EAS exhibits anti-photoaging potencies by preventing the reduction in HSP70 expression in UV-irradiated dermal fibroblasts.
METHODS: NHDFs were treated with 1 mg/mL of EAS or dextrin (vehicle control) prior to UV-B irradiation (20 mJ/cm2). After culturing NHDFs for different time periods, HSP70 mRNA and protein levels were analyzed using real-time polymerase chain reaction and western blotting, respectively.
RESULTS: UV-B-irradiated NHDFs showed reduced HSP70 mRNA levels after 1-6 h of culture, which were recovered after 24 h of culture. Treatment with EAS alone for 24 h increased HSP70 mRNA levels in the NHDFs, but the increase was not reflected in its protein levels. On the other hand, pretreatment with EAS abolished the UV-B irradiation-induced reduction in HSP70 expression at both mRNA and protein levels. These results suggest that EAS is capable to preserve HSP70 quantity in UV-B-irradiated NHDFs.
CONCLUSIONS: EAS exhibits anti-photoaging potencies by preventing the reduction in HSP70 expression in UV-irradiated dermal fibroblasts.
摘要：
背景：热休克蛋白70（HSP70）对紫外线（UV）诱导的皮肤过早老化具有保护作用。芦笋茎（EAS）的标准化提取物是一种新颖而独特的功能食品，可诱导HSP70细胞表达。为了阐明EAS的抗光老化功效，我们检查了其对UV-B照射的正常人真皮成纤维细胞（NHDFs）中HSP70表达水平的影响。
方法:在UV-B照射(20mJ/cm2)之前,用lmg/mL的EAS或糊精(载体对照)处理NHDF。在不同时间段培养NHDF后，使用实时聚合酶链反应和蛋白质印迹分析HSP70mRNA和蛋白质水平，分别。
结果：UV-B照射的NHDFs在培养1-6小时后显示HSP70mRNA水平降低，培养24小时后恢复。单独使用EAS治疗24小时可增加NHDFs中的HSP70mRNA水平，但这种增加并没有反映在蛋白质水平上。另一方面,EAS预处理消除了UV-B辐射诱导的mRNA和蛋白质水平的HSP70表达降低。这些结果表明EAS能够在UV-B辐照的NHDF中保持HSP70的量。
结论：EAS通过防止紫外线照射的真皮成纤维细胞中HSP70表达的减少而表现出抗光衰效力。
方法:在UV-B照射(20mJ/cm2)之前,用lmg/mL的EAS或糊精(载体对照)处理NHDF。在不同时间段培养NHDF后，使用实时聚合酶链反应和蛋白质印迹分析HSP70mRNA和蛋白质水平，分别。
结果：UV-B照射的NHDFs在培养1-6小时后显示HSP70mRNA水平降低，培养24小时后恢复。单独使用EAS治疗24小时可增加NHDFs中的HSP70mRNA水平，但这种增加并没有反映在蛋白质水平上。另一方面,EAS预处理消除了UV-B辐射诱导的mRNA和蛋白质水平的HSP70表达降低。这些结果表明EAS能够在UV-B辐照的NHDF中保持HSP70的量。
结论：EAS通过防止紫外线照射的真皮成纤维细胞中HSP70表达的减少而表现出抗光衰效力。
