在仪器分析之前,富含淀粉和富含蛋白质的谷物样品通常需要繁琐的样品制备步骤。本研究开发了一种小型化离心集成冷致相分离(CIPS)方法,以方便样品制备。一个小型离心管(2毫升)和一个低温离心机,两者都很容易接近,组成系统的基本组成部分。与需要逐步提取的常规样品制备方法不同,富集,净化,和离心,这种离心集成CIPS方法可以在低温离心机下通过一步组合方案进行.作为一项概念验证研究,从谷物中提取伏马菌素和曲霉毒素具有令人满意的回收率和富集因子。通过使用tSIM采集模式结合LC-HRMS产生了一种灵敏和选择性的定量方法,具有良好的线性(R2>0.998),准确度(82.9-106.5%),和精度(<13.4%)。这个策略很方便,低成本,可重复,易于半自动化,进一步扩大其他酸性真菌毒素的提取潜力。
Starches-rich and protein-rich cereal samples commonly need tedious sample preparation steps before instrumental analysis. This
study developed a miniaturized centrifugal integrated cold-induced phase separation (CIPS) method for convenient sample preparation. A small-sized centrifuge tube (2 mL) and a low-temperature centrifuge, both of which are easily accessible, make up the basic components of the system. Unlike conventional sample preparation methods that need a step-by-step extraction, enrichment, purification, and centrifugation, this centrifugal integrated CIPS method can be performed by a one-step combination protocol under a low-temperature centrifuge. As a proof-of-concept
study, satisfactory recoveries and enrichment factors were demonstrated for the extraction of fumonisins and ochratoxins from cereals. A sensitive and selective quantification method was yielded by combining LC-HRMS using tSIM acquisition mode, with good linearity (R2 > 0.998), accuracy (82.9-106.5%), and precision (<13.4%). This strategy is convenient, low-cost, repeatable, and easy to semi-automate, further expanding the extraction potential for other acidic mycotoxins.